Neuroprotective Effect of Steamed and Fermented Codonopsis lanceolata.
- Author:
Jin Bae WEON
1
;
Bo Ra YUN
;
Jiwoo LEE
;
Min Rye EOM
;
Hyun Jeong KO
;
Hyeon Yong LEE
;
Dong Sik PARK
;
Hee Chul CHUNG
;
Jae Youn CHUNG
;
Choong Je MA
Author Information
1. Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon 200-701, Republic of Korea. cjma@kangwon.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Codonopsis lanceolata;
Steam and fermentation;
Neuroprotective activity;
Antioxidative activity;
Bax;
Caspase-3
- MeSH:
Asthma;
Caspase 3;
Cell Death;
Codonopsis*;
Glutathione;
Glutathione Reductase;
Herbal Medicine;
Lung;
Neuroprotective Agents*;
Oxidative Stress;
Palatine Tonsil;
Pharyngitis;
Steam*;
Tonsillitis
- From:Biomolecules & Therapeutics
2014;22(3):246-253
- CountryRepublic of Korea
- Language:English
-
Abstract:
Codonopsis lanceolata has been used as an herbal medicine for several lung infl ammatory diseases, such as asthma, tonsillitis, and pharyngitis. Previously, we showed the neuroprotective effect of steamed and fermented C. lanceolata (SFC) in vitro and in vivo. In the current study, the treatment of HT22 cells with SFC decreased glutamate-induced cell death, suggesting that SFC protected HT22 cells from glutamate-induced cytotoxicity. Based on these, we sought to elucidate the mechanisms of the neuroprotective effect of SFC by measuring the oxidative stress parameters and the expression of Bax and caspase-3 in HT22 cells. SFC reduced contents of ROS, Ca2+ and NO. Moreover, SFC restored contents of glutathione and glutathione reductase as well as inhibited Bax and caspase-3 activity in HT22 cells. These results indicate that steamed and fermented C. lanceolata (SFC) extract protected HT22 cells by anti-oxidative effect and inhibition of the expression of Bax and caspase-3.
