GC-MS analysis of volatile oil components of Mastiche and Olibanum and study on antibacterial activity of Helicobacter pylori.
10.19540/j.cnki.cjcmm.20201214.304
- Author:
Ling YANG
1
;
Long-Fei LIN
2
;
Yu-Ling LIU
2
;
Hui LI
2
;
Lu-Qi HUANG
3
Author Information
1. Academician Workstation,Jiangxi University of Traditional Chinese Medicine Nanchang 330004,China National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China.
2. Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China.
3. National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China.
- Publication Type:Journal Article
- Keywords:
GC-MS;
Helicobacter pylori;
Mastiche;
Olibanum;
in vitro bacteriostasis;
volatile oil
- MeSH:
Anti-Bacterial Agents/pharmacology*;
Frankincense;
Gas Chromatography-Mass Spectrometry;
Helicobacter pylori;
Monoterpenes/analysis*;
Oils, Volatile/pharmacology*
- From:
China Journal of Chinese Materia Medica
2021;46(5):1141-1147
- CountryChina
- Language:Chinese
-
Abstract:
The volatile oil from Mastiche and Olibanum medicinal materials was extracted by steam distillation, and the chemical components of the volatile oil were analyzed by GC-MS technology. The differences of the volatile oil components were compared and study on the Helicobacter pylori in vitro antimicrobial activitiy was conducted. The results showed that the yields of the volatile oil from Mastiche and Olibanum were 11.93% and 2.40%, respectively. A total of 46 compounds(91.31%) were identified from the volatile oil from Mastiche annd 35 compounds(92.49%) from Olibanum. The classification and comparison study of the components showed that the content of monoterpenes in the volatile oil from Mastiche was the highest(40.69%), followed by alcohols(28.48%); while the content of alcohols in the volatile oil from Olibanum was the highest(35.81%), followed by esters(24.92%). There were significant differences in the components of volatile oil from Mastiche and Olibanum, which might be one of the reasons for the difference in efficacy and application. In vitro bacteriostatic experiments showed that the minimum inhibitory concentration(MIC) of the volatile oil from Mastiche against H. pylori was 1 mg·mL~(-1), and the MIC of the volatile oil from Olibanum against H. pylori was more than 1 mg·mL~(-1). In combination with the results of the oil yield experiment, Mastiche had the advantage of inhibiting H. pylori activity. The research results provide scientific basis for the rational application of Mastiche and Olibanum.
