Identification of potential genes involved in biosynthesis of flavonoid and analysis of biosynthetic pathway in Fagopyrum dibotrys.
10.19540/j.cnki.cjcmm.20201121.102
- Author:
Xin WU
1
;
Chen-Kai WANG
2
;
Hai-Yan ZUO
2
;
Zhao-Hui CHEN
3
;
Sheng-Bing WU
4
;
Mei-Qi ZHOU
5
Author Information
1. School of Acupuncture and Massage,Anhui University of Chinese Medicine Hefei 230038,China Graduate School,Anhui University of Chinese Medicine Hefei 230038,China.
2. Graduate School,Anhui University of Chinese Medicine Hefei 230038,China.
3. School of Acupuncture and Massage,Anhui University of Chinese Medicine Hefei 230038,China.
4. Key Laboratory of Xin'an Medicine,Ministry of Education,Anhui University of Chinese Medicine Hefei 230038,China.
5. School of Acupuncture and Massage,Anhui University of Chinese Medicine Hefei 230038,China Bozhou Institute of Chinese Medicine,Anhui Academy of Traditional Chinese Medicine Bozhou 236800,China.
- Publication Type:Journal Article
- Keywords:
Fagopyrum dibotrys;
differentially expressed genes;
flavonoid;
transcriptome
- MeSH:
Biosynthetic Pathways/genetics*;
Fagopyrum;
Flavonoids;
Flowers;
Gene Expression Profiling;
Gene Expression Regulation, Plant;
Humans;
Transcriptome/genetics*
- From:
China Journal of Chinese Materia Medica
2021;46(5):1084-1093
- CountryChina
- Language:Chinese
-
Abstract:
In order to enrich the transcriptome data of Fagopyrum dibotrys plants, analyze the genes encoding key enzyme involved in flavonoid biosynthesis pathway, and mine their functional genes, in this study, we performed RNA sequencing analysis for the rhizomes, roots, flowers, leaves and stems of F. dibotrys on the BGISEQ-500 sequencing platform. After de novo assembly of transcripts, a total of 205 619 unigenes were generated and 132 372 unigenes were obtained and annotated into seven public databases, of which, 81 327 unigenes were mapped to the GO database and most of the unigenes were annotated in cellular process, biological regulation, binding and catalytic activity. Besides, 86 922 unigenes were enriched in 136 pathways using KEGG database' and we identified 82 unigenes that encodes key enzymes involved in flavonoid biosynthesis. Comparing rhizome with root, flower, leaf or stem in F. dibotrys, 27 962 co-expressed differentially expressed genes(DEGs) were obtained. Among them, 23 515 DEGs of rhizome tissue-specific were enriched into 132 pathways and 13 unigenes were significantly enriched in biosynthesis of flavone and flavonol. In addition, we also identified 3 427 unigenes encoding 60 transcription factor(TFs) families as well as four unigenes encoding bHLH TFs were enriched in flavonoid biosynthesis. Our results greatly enriched the transcriptome database of plants, provided a reference for the analysis of key enzymes involved in flavonoid biosynthesis in plants, and will facilitate the study of the functions and regulatory mechanisms of key enzymes involved in flavonoid biosynthesis in F. dibotrys at the genetic level.
