Research progress in original species identification in industry chain of Rhei Radix et Rhizoma.
10.19540/j.cnki.cjcmm.20201207.601
- Author:
Ran-Jun LI
1
;
Tian-Yi XIN
2
;
Liang-Ke SONG
3
;
Hai-Xia YAN
4
;
Hai LIAO
3
;
Jia-Yu ZHOU
3
;
Jing-Yuan SONG
5
Author Information
1. Key Laboratory of Chinese Medicine Resources Conservation,State Administration of Traditional Chinese Medicine of the People's Republic of China,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100193,China School of Life and Science,Southwest Jiaotong University Chengdu 610031,China.
2. Key Laboratory of Chinese Medicine Resources Conservation,State Administration of Traditional Chinese Medicine of the People's Republic of China,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100193,China.
3. School of Life and Science,Southwest Jiaotong University Chengdu 610031,China.
4. Beijing Key Laboratory of Analysis and Evaluation on Chinese Medicine,NMPA (National Medical Products Administration) Key Laboratory for Quality Evaluation of Traditional Chinese Medicine (Traditional Chinese Patent Medicine),Beijing Institute for Drug Control (Beijing Center for Health Food And Cosmetics Control) Beijing 102206,China.
5. Key Laboratory of Chinese Medicine Resources Conservation,State Administration of Traditional Chinese Medicine of the People's Republic of China,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100193,China Engineering Research Center of Chinese Medicine Resource of Ministry of Education Beijing 100193,China.
- Publication Type:Journal Article
- Keywords:
DNA barcoding;
Rhei Radix et Rhizoma;
decoction pieces;
original species identification;
traditional Chinese patent medicine
- MeSH:
Animals;
Anthraquinones;
Drugs, Chinese Herbal;
Plant Roots;
Rheum;
Rhizome
- From:
China Journal of Chinese Materia Medica
2021;46(5):1060-1066
- CountryChina
- Language:Chinese
-
Abstract:
Rhei Radix et Rhizoma is a kind of commonly used Chinese medicinal materials. Due to the overharvesting, the wild resource is endangering. Large market demand caused severely adulterant of commercial Rhei Radix et Rhizoma medicinal materials and decoction pieces. This manuscript reviewed the advances of the original species authentication in the industrial chain of Rhei Radix et Rhizoma during the latest decade, including characteristics and microscopic features, phytochemical analysis on anthraquinones, and molecular authentication based on DNA barcoding. Accordingly, an original species authentication route for the industrial chain of Rhei Radix et Rhizoma was summarized:(1)the identification of seeds and seedlings by DNA barcoding;(2) the selection of high variable sites based on the chloroplast genome;(3)biomonitoring of the Rhei Radix et Rhizoma medicinal materials and decoction pieces by two-dimensional DNA barcode;(4)traceability of Chinese patent medicines by third-generation sequencing. In conclusion, the combination of molecular identification and traditional identification methods provides a new idea for the identification of the original species of Rhei Radix et Rhizoma in the industrial chain and a essential guidance for the research of drug safety and efficacy of Rhei Radix et Rhizoma.
