Study on preparation extraction process of Linggui Zhugan Decoction.
10.19540/j.cnki.cjcmm.20201022.307
- Author:
Zheng-Gen LIAO
1
;
Qi-Li YUAN
1
;
Xin-Li LIANG
1
;
Hai-Bo DING
1
;
Qie-Ying JIANG
2
Author Information
1. Key Laboratory of Modern Preparation of Traditional Chinese Medicine under Ministry of Education,Jiangxi University of Traditional Chinese Medicine Nanchang 330004,China.
2. Laboratory Animal Research Center for Science and Technology,Jiangxi University of Traditional Chinese Medicine Nanchang 330004,China.
- Publication Type:Journal Article
- Keywords:
Linggui Zhugan Decoction;
classical prescription;
extraction process;
solid material reference
- MeSH:
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
Glycyrrhizic Acid;
Oils, Volatile;
Quality Control;
Reference Standards
- From:
China Journal of Chinese Materia Medica
2021;46(4):830-836
- CountryChina
- Language:Chinese
-
Abstract:
To verify the appropriate preparation process of extracts for the solid substance benchmark of Linggui Zhugan Decoction. The extracts were prepared by different preparation processes, namely the traditional process(process 1), the extract combined with volatile oil separated from traditional process extract liquid(process 2), the modern secondary reflux extraction process(process 3) and the process that volatile oil was extracted first, then prepared according to the traditional process, and combined with extract(process 4); based on the characteristic spectrum, index components of cinnamaldehyde, glycyrrhizin, ammonium glycyrrhizinate, cinnamic acid, and the dry extract rate of process 1, the differences and similarities of four extracts were compared. The results showed that the similarity of the characteristic spectrum of process 2, process 4 and process 1 were all greater than 0.97, while there was no significant difference for the content of 4 quality control components and dry extract rate; the similarity of the characteristic spectrum of process 3 and process 1 was 0.91, the absolute peak area of 13 out of 21 peaks and the relative peak area of 7 peaks increased significantly, and the content of 3 out of 4 quality control components and dry extract rate also significantly increased. In conclusion, the material standards of extracts by the process 2 and 4 are consistent with that of the traditional process, so the two processes are suitable.
