Molecular mechanism study of Shouhui Tongbian Capsules on promoting energy metabolism of gastrointestinal stromal cells.
10.19540/j.cnki.cjcmm.20201209.401
- Author:
Xiao-Wen ZHANG
1
;
Shu-Ze ZHENG
1
;
Xin LI
2
;
Cheng-Hong SUN
2
;
Ke-Wu ZENG
1
Author Information
1. State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Beijing 100191, China.
2. State Key Laboratory of Generic Manufacture Technology of Chinese Traditional Medicine,Lunan Pharmaceutical Group Co., Ltd. Linyi 276006, China.
- Publication Type:Journal Article
- Keywords:
Shouhui Tongbian Capsules;
energy metabolism;
gastrointestinal stromal cells;
isocitrate dehydrogenase;
mitochondrial function
- MeSH:
Capsules;
Cell Line, Tumor;
Energy Metabolism;
Gastrointestinal Stromal Tumors;
Humans;
Proto-Oncogene Proteins c-kit/metabolism*;
Stromal Cells/metabolism*
- From:
China Journal of Chinese Materia Medica
2021;46(3):526-531
- CountryChina
- Language:Chinese
-
Abstract:
Mechanism study was performed to explore how Shouhui Tongbian Capsules promotes energy metabolism of gastrointestinal stromal cells. In this study, gastrointestinal stromal cells line GIST-882 was used as the model to explore energy metabolism regulation effects of Shouhui Tongbian Capsules extract(10, 20, 50 and 100 μg·mL~(-1)) by measuring the cell proliferation, ATP level, mitochondrial membrane potential, and mitochondrial isocitrate dehydrogenase activity. Meanwhile, Western blot was used to detect the proteins expression of SCF/c-Kit and CDK2/cyclin A signaling pathways. Our results showed that Shouhui Tongbian Capsules promoted cell proliferation and increased ATP level of gastrointestinal stromal cells. In addition, Shouhui Tongbian Capsules obviously improved mitochondrial structural integrity, and increased mitochondrial membrane potential in GIST-882 cells. Mechanism study revealed that Shouhui Tongbian Capsules increased mitochondrial isocitrate dehydrogenase activity and up-regulated the proteins expression of SCF/c-Kit and CDK2/cyclin A signaling pathways. Collectively, our study indicated that Shouhui Tongbian Capsules promoted the energy metabolism for gastrointestinal stromal cells proliferation by activating mitochondrial isocitrate dehydrogenase to induce ATP production, as well as activating SCF/c-Kit and CDK2/cyclin A signaling pathways.
