Study on internal and external quality control methods of Platycladi Cacumen Carbonisata based on QAMS and color recognition.
10.19540/j.cnki.cjcmm.20200904.301
- Author:
Dian-Hua SHI
1
;
Yan-Peng DAI
2
;
Ben-Zheng SU
2
;
Li-Li SUN
2
;
Xue-Lan ZHANG
3
;
Jun ZHANG
2
Author Information
1. Shandong Academy of Chinese Medicine Ji'nan 250014, China Shandong University of Traditional Chinese Medicine Ji'nan 250355, China.
2. Shandong Academy of Chinese Medicine Ji'nan 250014, China.
3. Shandong University of Traditional Chinese Medicine Ji'nan 250355, China.
- Publication Type:Journal Article
- Keywords:
Platycladi Cacumen Carbonisata;
color recognition;
quality standard;
quantitative analysis of multi-components by single marker
- MeSH:
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
Flavonoids;
Hemostatics;
Quality Control
- From:
China Journal of Chinese Materia Medica
2020;45(24):5996-6002
- CountryChina
- Language:Chinese
-
Abstract:
In order to effectively solve the over-processing problem of Platycladi Cacumen Carbonisata, which was commonly used as a hemostatic drug in clinical application, we used the quantitative analysis method of multi-components by single marker(QAMS) in this study, with quercetin as internal reference to simultaneously determine the content of six flavonoids which can be used to control the internal quality of Platycladi Cacumen Carbonisata. Based on the comparison of QAMS and external standard method(ESM) results, the limit standards of contents were established as follows: isoquercitroside ≥0.002 0%, quercitroside ≥0.050%, quercetin ≥0.030%, kaempferol and amentoflavone both ≥0.010%, hinokiflavone ≥0.050%. Based on the color detection of Platycladi Cacumen and Platycladi Cacumen Carbonisata with different processing degrees, the law of influence of different processing degrees on the color of Platycladi Cacumen Carbonisata was found. A new external quality standard of Platycladi Cacumen Carbonisata was established by fitting curve of color recognition for the external quality control, based on which the standard ranges of ΔL~*, Δb~* and ΔE were-50.00--44.00, 6.00-11.00 and 45.00-50.00 respectively. Effective combination of established internal and external quality control standards by this study can be used to evaluate the processing degree and quality of Platycladi Cacumen Carbonisata more comprehensively and objectively, which can guarantee its clinical efficacy. At the same time, this study also provides reference and basis for further improving the quality control standard of Platycladi Cacumen Carbonisata.
