Improvement researches on quality standard of Anemarrhenae Rhizoma and its raw processed products.
10.19540/j.cnki.cjcmm.20200629.202
- Author:
Qi HUANG
1
;
Zhi-Yuan SHAO
2
;
De-Ling WU
1
;
Dai-Yin PENG
1
;
Chuan-Shan JIN
1
;
Qi-Ling ZHANG
3
;
Kai MA
4
Author Information
1. College of Pharmacy, Anhui University of Chinese Medicine Hefei 230012, China Anhui Province Key Laboratory of Traditional Chinese Medicine Decoction Pieces of New Manufacturing Technology Heifei 230012, China.
2. College of Pharmacy, Anhui University of Chinese Medicine Hefei 230012, China.
3. Anhui Guangyintang Traditional Chinese Medicine Company Bozhou 236800, China.
4. Bozhou Yonggang Traditional Chinese Medicine Company Bozhou 236800, China.
- Publication Type:Journal Article
- Keywords:
Anemarrhenae Rhizoma;
TLC identification;
content determination;
fingerprint;
quality standard
- MeSH:
Anemarrhena;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
Reference Standards;
Rhizome
- From:
China Journal of Chinese Materia Medica
2020;45(23):5701-5711
- CountryChina
- Language:Chinese
-
Abstract:
This study is to improve the quality standard and supply the scientific basis for Anemarrhenae Rhizoma and its raw processed products. Steroidal saponin including timosaponin BⅡ, timosaponin AⅢ and flavonoids including neomangiferin and mangiferin were selected as the indicative components. Silica gel G thin layer chromatography(TLC) and polyamide TLC were used to detect the two types of compounds, respectively. The contents of timosaponin BⅡ and timosaponin AⅢ were determined by HPLC-ELSD and the content of neomangiferin, mangiferin and isomangiferin were determined by HPLC-UV. Moisture, total ash and acid insoluble ash were determined according to Chinese Pharmacopoeia(2015 edition). And 80% ethanol was selected as the solvent and the content determination of total extract were determined. The fingerprints of Anemarrhenae Rhizoma and its raw processed products were established by HPLC-UV and HPLC-ELSD. The results showed that the methods of TLC and HPLC have been successfully stablished. There are 2 and 3 peaks which have been identified by HPLC-ELSD and HPLC-UV, respectively. The HPLC fingerprint methods are specific and can be used to identify and quality control for Anemarrhenae Rhizoma and its raw processed products in the mass. Comparing to Chinese Pharmacopoeia(2015 edition), the TLC identification and content determination were revised and the total extract determination and HPLC fingerprints were added in the present study. Our results can be used as the scientific basis of quqlity control for Anemarrhenae Rhizoma and its raw processed products.
