UPLC-Q-TOF-MS analysis on chemical constituents of classical prescription Huangqi Guizhi Wuwu Tang standard decoction.
10.19540/j.cnki.cjcmm.20200915.301
- Author:
Ru-Ling XU
1
;
Jun-Ting FAN
2
;
Hui-Min DONG
3
;
Xiao-Qing CHEN
3
;
Jin-di XU
3
;
Jie-Ge HUO
3
;
Jian-Ming JU
3
Author Information
1. Anhui University of Chinese Medicine Hefei 230031, China Jiangsu Province Academy of Traditional Chinese Medicine Nanjing 210028, China.
2. School of Pharmacy, Nanjing Medical University Nanjing 211166, China.
3. Jiangsu Province Academy of Traditional Chinese Medicine Nanjing 210028, China Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine Nanjing 210028, China.
- Publication Type:Journal Article
- Keywords:
Huangqi Guizhi Wuwu Tang standard decoction;
UPLC-Q-TOF-MS;
chemical constituent;
classical prescription
- MeSH:
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
Flavonoids/analysis*;
Glycosides;
Prescriptions;
Reference Standards
- From:
China Journal of Chinese Materia Medica
2020;45(23):5614-5630
- CountryChina
- Language:Chinese
-
Abstract:
UPLC-Q-TOF-MS technology was used to analyze the chemical constituents from classical prescription Huangqi Guizhi Wuwu Tang standard decoction. Acquity HSS T3 column(2.1 mm × 100 mm, 1.8 μm) was used as the chromatographic column, with 0.1% formic acid solution-0.1% formic acid acetonitrile as the mobile phase for gradient elution. The volume flow rate was 0.4 mL·min~(-1) and the column temperature was 40 ℃. Mass spectrometry data of Huangqi Guizhi Wuwu Tang standard decoction were collected in positive and negative ion modes. The chemical constituents from classical prescription Huangqi Guizhi Wuwu Tang standard decoction were analyzed and identified by Masslynx 4.1 software combined with SciFinder database, comparison with reference mate-rials, mass spectrometry data analysis and reference to relevant literature. A total of 110 compounds were analyzed and identified, including 33 flavonoids, 14 monoterpene glycosides, 8 triterpenoids, 8 gingerols, 17 phenylpropanoids, 12 organic acids, 7 amino acids and 11 other compounds. The results of this study provide an experimental basis for the further research on the substance basis and quality control of Huangqi Guizhi Wuwu Tang standard decoction.
