Comparative transcriptomics analysis of Citrus medica var. sarcodactylis at different developmental stages.
10.19540/j.cnki.cjcmm.20200722.101
- Author:
Yuan PAN
1
;
Da-Xia CHEN
1
;
Li LONG-YUN
1
Author Information
1. Chongqing Engineering Research Center for Fine Variety Breeding Techniques of Chinese Materia Medica,Chongqing Sub-center of National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Chongqing Academy of Chinese Materia Medica Chongqing 400065, China.
- Publication Type:Journal Article
- Keywords:
Citrus medica var.sarcodactylis;
active ingredients;
development period;
gene expression
- MeSH:
Citrus/genetics*;
Computational Biology;
Fruit/genetics*;
Gene Expression Profiling;
Gene Expression Regulation, Plant;
High-Throughput Nucleotide Sequencing;
Transcriptome
- From:
China Journal of Chinese Materia Medica
2020;45(21):5169-5176
- CountryChina
- Language:Chinese
-
Abstract:
In order to study the transcriptional differences of Citrus medica var. sarcodactylis at different developmental stages, we explored the genes regulating the biosynthesis of the effective components. In this study, Illumina Hiseq 4 000 high-throughput sequencing technology was used to sequence the transcriptome of C. medica var. sarcodactylis at different developmental stages, 121 235 unigenes were obtained with an average length of 2 434 bp, 3 379 different genes were obtained using DESeq screening, which mainly connected to biological processes such as signal transmission, biological regulation, and metabolic processes, and enriched in metabolic pathways such as starch, sucrose metabolism, phenylpropanoid biosynthesis, and flavonoid biosynthesis. Further dynamic comparison of biosynthesis related genes of active ingredients: the expression levels of PAL, CHI, CYP75B1, ZDS, 4CL and FLS gradually increased as the fruit turned from green to yellow; the expressions of COMT, F3H and CYP73A increased at first and then decreased; CCR, HCT and HRP were down-regulated whereas up-regulated. This study provides references for further excavation of key genes in the biosynthesis of active components, as well as biopathway analysis of active components for C. medica var. sarcodactylis.
