Effects of Vitrification with Self-made Carriers and Slow Programmed Freezing on Ovarian Tissue of Sheep.
- Author:
Yan Ting CUI
1
;
Lu Kai YANG
1
;
Jin LIU
1
;
Yi Long HAN
1
;
Chao CHEN
1
;
Xiao Hui DENG
1
;
Li Gang JIANG
1
Author Information
- Publication Type:Journal Article
- Keywords: carrier; ovarian tissue; sheep; vitrification
- MeSH: Animals; Cryopreservation; Female; Freezing; Ovarian Follicle; Ovary; Sheep; Vitrification
- From: Acta Academiae Medicinae Sinicae 2021;43(2):193-198
- CountryChina
- Language:Chinese
- Abstract: Objective To investigate the effects of self-made carriers on the cryopreservation of ovarian tissue of sheep. Methods Thirty-two ovaries were randomly assigned to fresh group,programmed freezing group,self-made carrier I vitrification group,and self-made carrier Ⅱ vitrification group.The morphology,proliferation,apoptosis,and estrogen level of the ovarian tissue in each group were observed. Results After cryopreservation,the morphology normal rate of the primordial follicles in programmed freezing group,self-made carrier I vitrification group,and self-made carrier Ⅱ vitrification group were 74.2%,72.8%,and 72.3%,respectively,lower than that(83.7%)in the fresh group(χ
