Therapeutic Effect of SPK1 Gene Transfected Adipose Derived Mesenchymal Stem Cells on Experimental Autoimmune Encephalomyelitis Mice and Its Effect on T Helper Cell 17/Regulatory T Cells Balance.
10.3881/j.issn.1000-503X.11888
- Author:
Tao ZHOU
1
;
Chao Ping XU
1
;
Ying XIAO
1
;
Qian ZHANG
1
;
Li LI
1
Author Information
1. Department of Rheumatology and Immunology,Wuhan Puren Hospital,Wuhan University of Science and Technology,Wuhan 430081,China.
- Publication Type:Journal Article
- Keywords:
T helper cell 17/regulatory T cells balance;
adipose derived mesenchymal stem cells;
experimental autoimmune encephalomyelitis;
multiple sclerosis;
sphingosine kinase 1 gene transfection
- MeSH:
Adipose Tissue/cytology*;
Animals;
Cytokines;
Encephalomyelitis, Autoimmune, Experimental/therapy*;
Interleukin-17;
Mesenchymal Stem Cell Transplantation;
Mesenchymal Stem Cells/cytology*;
Mice;
Mice, Inbred C57BL;
Phosphotransferases (Alcohol Group Acceptor)/genetics*;
T-Lymphocytes, Regulatory/cytology*;
Th17 Cells/cytology*;
Transfection
- From:
Acta Academiae Medicinae Sinicae
2020;42(6):755-765
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the therapeutic effect of SPK1 gene transfected adipose derived mesenchymal stem cells(ADMSC)on experimental autoimmune encephalomyelitis mice and the effect on T helper cell 17(Th17)/regulatory T(Treg) cells balance. Methods EAE was induced by myelin oligodendrocyte glycoprotein 35-55 in mice.Totally 44 mice were randomly divided into four groups:normal control group(NC group),model group(EAE group),ADMSC group,and ADMSC-SPK1 group.Forty days after injection,the pathological changes of brain and spinal cord,Th17/Treg-related inflammatory markers in brain tissue,expressions of interleukin-17A(IL-17A)and forkhead box protein p3(Foxp3)in brain and spinal cord tissue,and flow cytometric results of spleen immune cells were detected. Results Forty days after the injection,serious inflammatory cell infiltration and demyelination occurred in the brain and spinal cord of EAE group,whereas demyelination and axonal injury were improved in ADMSC group and ADMSC-SPK1 group.Compared with EAE group,the ADMSC group and ADMSC-SPK1 group had significantly improved levels of IL-17A(