Advances in metabolic engineering of non-conventional yeasts.

Author: Liqiu SU ¹ ; Ge ZHANG ¹ ; Zhen YAO ¹ ; Peixin LIANG ¹ ; Zongjie DAI ¹ ; Qinhong WANG ¹
Author Information

1. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
Publication Type:Review
Keywords: bio-based manufacturing; gene editing; metabolic engineering; non-conventional yeasts
MeSH: Gene Editing; Metabolic Engineering; Pichia/genetics*; Synthetic Biology; Yarrowia/genetics*; Yeasts
From: Chinese Journal of Biotechnology 2021;37(5):1659-1676
CountryChina
Language:Chinese
Abstract: Over the past 30 years, Yarrowia lipolytica, Kluyveromyces, Pichia, Candida, Hansenula and other non-conventional yeasts have attracted wide attention because of their desirable phenotypes, such as rapid growth, capability of utilizing multiple substrates, and stress tolerance. A variety of synthetic biology tools are being developed for exploitation of their unique phenotypes, making them potential cell factories for the production of recombinant proteins and renewable bio-based chemicals. This review summarizes the gene editing tools and the metabolic engineering strategies recently developed for non-conventional yeasts. Moreover, the challenges and future perspectives for developing non-conventional yeasts into efficient cell factories for the production of useful products through metabolic engineering are discussed.