Recent advances in developing enabling technologies for Corynebacterium glutamicum metabolic engineering.

Author: Yu WANG ¹ ; Ping ZHENG ¹ ; Jibin SUN ¹
Author Information

1. Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin Institute of Industrial Biotechnology, Tianjin 300308, China.
Publication Type:Review
Keywords: Corynebacterium glutamicum; adaptive laboratory evolution; biosensor; expression regulation; genome editing; metabolic engineering
MeSH: Amino Acids; Biotechnology; Corynebacterium glutamicum/genetics*; Gene Editing; Metabolic Engineering
From: Chinese Journal of Biotechnology 2021;37(5):1603-1618
CountryChina
Language:Chinese
Abstract: Corynebacterium glutamicum is an important workhorse of industrial biotechnology, especially for amino acid bioindustry. This bacterium is being used to produce various amino acids at a level of over 6 million tons per year. In recent years, enabling technologies for C. glutamicum metabolic engineering have been developed and improved, which accelerated construction and optimization of microbial cell factoriers, expanding spectra of substrates and products, and facilitated basic researches on C. glutamicum. With these technologies, C. glutamicum has become one of the ideal microbial chasses. This review summarizes recent key technological developments of enabling technologies for C. glutamicum metabolic engineering and focuses on establishment and applications of CRISPR-based genome editing, gene expression regulation, adaptive laboratory evolution, and biosensor technologies.