Metabolic engineering tools for Saccharomyces cerevisiae.

Lihong JIANG; Chang DONG; Lei HUANG; Zhinan XU; Jiazhang LIAN

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Metabolic engineering tools for Saccharomyces cerevisiae.

Author: Lihong JIANG ¹ ; Chang DONG ¹ ; Lei HUANG ¹ ; Zhinan XU ¹ ; Jiazhang LIAN ¹
Author Information

1. Key Laboratory of Biomass Chemical Engineering, Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, Zhejiang, China.
Publication Type:Review
Keywords: Saccharomyces cerevisiae; genome evolution; metabolic engineering; synthetic biology; systems biology
MeSH: Computational Biology; Metabolic Engineering; Saccharomyces cerevisiae/genetics*; Synthetic Biology
From: Chinese Journal of Biotechnology 2021;37(5):1578-1602
CountryChina
Language:Chinese
Abstract: Since its birth in the early 1990s, metabolic engineering technology has gone 30 years rapid development. As one of the preferred chassis for metabolic engineering, S. cerevisiae cells have been engineered into microbial cell factories for the production of a variety of bulk chemicals and novel high value-added bioactive compounds. In recent years, synthetic biology, bioinformatics, machine learning and other technologies have also greatly contributed to the technological development and applications of metabolic engineering. This review summarizes the important technological development for metabolic engineering of S. cerevisiae in the past 30 years. Firstly, classical metabolic engineering tools and strategies were reviewed, followed by reviewing systems metabolic engineering and synthetic biology driven metabolic engineering approaches. The review is concluded with discussing future perspectives for metabolic engineering of S. cerevisiae in the light of state-of-the-art technological development.