Construction of Escherichia coli cell factories.
- Author:
Yong YU
1
;
Xinna ZHU
1
;
Changhao BI
1
;
Xueli ZHANG
1
Author Information
- Publication Type:Review
- Keywords: Escherichia coli; metabolic engineering; metabolic pathway; pathway optimization
- MeSH: Artificial Intelligence; Escherichia coli/genetics*; Gene Editing; Metabolic Engineering; Metabolic Networks and Pathways/genetics*
- From: Chinese Journal of Biotechnology 2021;37(5):1564-1577
- CountryChina
- Language:Chinese
- Abstract: As an important model industrial microorganism, Escherichia coli has been widely used in pharmaceutical, chemical industry and agriculture. In the past 30 years, a variety of new strategies and techniques, including artificial intelligence, gene editing, metabolic pathway assembly, and dynamic regulation have been used to design, construct, and optimize E. coli cell factories, which remarkably improved the efficiency for biotechnological production of chemicals. In this review, three key aspects for constructing E. coli cell factories, including pathway design, pathway assembly and regulation, and optimization of global cellular performance, are summarized. The technologies that have played important roles in metabolic engineering of E. coli, as well as their future applications, are discussed.
