Thirty years development of ¹³C metabolic flux analysis: a review.
- Author:
Ruilian YAO
1
Author Information
- Publication Type:Review
- Keywords: biomedicine; industrial biotechnology; metabolic engineering; ¹³C metabolic flux analysis
- MeSH: Amino Acids; Carbon Isotopes; Isotope Labeling; Metabolic Engineering; Metabolic Flux Analysis; Models, Biological
- From: Chinese Journal of Biotechnology 2021;37(5):1510-1525
- CountryChina
- Language:Chinese
- Abstract: ¹³C metabolic flux analysis (¹³C-MFA) enables the precise quantification of intracellular metabolic reaction rates by analyzing the distribution of mass isotopomers of proteinogenic amino acids or intracellular metabolites through ¹³C labeling experiments. ¹³C-MFA has received much attention as it can help systematically understand cellular metabolic characteristics, guide metabolic engineering design and gain mechanistic insights into pathophysiology. This article reviews the advances of ¹³C-MFA in the past 30 years and discusses its potential and future perspective, with a focus on its application in industrial biotechnology and biomedicine.
