Application of SPR protein chip in screening for imported malaria.
- Author:
Fan CHEN
1
;
Jian'an HE
2
;
Ruiling DONG
2
;
Fan YANG
1
;
Houming LIU
3
;
Dayong GU
4
;
Wei WANG
4
Author Information
- Publication Type:Journal Article
- Keywords: malaria; protein chip; screening; surface plasmon resonance
- MeSH: Antibodies; China; Humans; Malaria/diagnosis*; Protein Array Analysis; Surface Plasmon Resonance
- From: Chinese Journal of Biotechnology 2021;37(4):1360-1367
- CountryChina
- Language:Chinese
- Abstract: Imported malaria has become a major risk factor for malaria prevention and control in China. How to screen malaria quickly for people entering China is an urgent problem to be solved. Protein microarrays are widely used in high-throughput screening and diagnosis. In this study, surface plasmon resonance (SPR) technique for malaria detection was established by using the specific adsorption surface treated by polyethylene glycol polymer, and the malaria specific antigen HRP2 was used as capture probe. The optimal concentration of antigen, sensitivity and specificity of detection, as well as anti-interference ability of the chip were analyzed. The SPR protein chip was applied to detect specific antibodies of malignant malaria in serum with the advantage of label-free, instant and fast. Compared with fluorescence quantitative PCR, there were no significant difference in sensitivity and specificity between the two methods. This study lays a foundation for further development of protein microarray for malaria typing identification, and it is conducive to the rapid screening of malaria for people entering.
