Expression and function analysis of FaCO gene in Festuca arundinacea.

Xi Chen; Ying Chen; Xiaoxia Liu; Jianhong Shu; Xiaoli Wang; Degang Zhao

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Expression and function analysis of FaCO gene in Festuca arundinacea.

Author: Xi CHEN ¹ ; Ying CHEN ¹ ; Xiaoxia LIU ¹ ; Jianhong SHU ¹ ; Xiaoli WANG ¹ ; Degang ZHAO ¹
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1. Guizhou Institute of Prataculture, Guizhou Academy of Agricultural Sciences, Guiyang 550006, Guizhou, China.
Publication Type:Journal Article
Keywords: expression analysis; photoperiodic regulation gene; tall fescue; vector construction
MeSH: Arabidopsis/metabolism*; Arabidopsis Proteins/genetics*; Festuca/metabolism*; Flowers/genetics*; Gene Expression Regulation, Plant; Photoperiod
From: Chinese Journal of Biotechnology 2021;37(4):1324-1333
CountryChina
Language:Chinese
Abstract: Photoperiod plays an important role in transformation from vegetative growth to reproductive growth in plants. CONSTANS (CO), as a unique gene in the photoperiod pathway, responds to changes of day length to initiate flowering in the plant. In this study, the expression level of FaCONSTANS (FaCO) gene under long-day, short-day, continuous light and continuous darkness conditions was analyzed by real-time quantitative PCR. We constructed the over-expression vector p1300-FaCO and infected into Arabidopsis thaliana by Agrobacterium-mediated method. We constructed the silencing vector p1300-FaCO-RNAi and infected into Festuca arundinacea by Agrobacterium-mediated method. The expression of FaCO gene was regulated by photoperiod. The over-expression of FaCO promoted flowering in wild type of Arabidopsis thaliana under long day condition and rescued the late flowering phenotype in co-2 mutant of Arabidopsis thaliana. Silencing FaCO gene in Festuca arundinacea by RNAi showed late-flowering phenotype or always kept in the vegetative growth stage. Our understanding the function of FaCO in flowering regulation will help further understand biological function of this gene in Festuca arundinacea.