Progress and perspective on development of non-model industrial bacteria as chassis cells for biochemical production in the synthetic biology era.

Yongfu Yang; Binan Geng; Haoyue Song; Mimi HU; Qiaoning HE; Shouwen Chen; Fengwu Bai; Shihui Yang

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Progress and perspective on development of non-model industrial bacteria as chassis cells for biochemical production in the synthetic biology era.

Author: Yongfu YANG ¹ ; Binan GENG ¹ ; Haoyue SONG ¹ ; Mimi HU ¹ ; Qiaoning HE ¹ ; Shouwen CHEN ¹ ; Fengwu BAI ² ; Shihui YANG ¹
Author Information

1. State Key Laboratory of Biocatalysis and Enzyme Engineering, Environmental Microbial Technology Center of Hubei Province, School of Life Sciences, Hubei University, Wuhan 430062, Hubei, China.
2. State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Publication Type:Review
Keywords: biosafety; chassis cells; industrial microorganism; microbial cell factories; non-model microorganisms; synthetic biology
MeSH: Bacteria/genetics*; Metabolic Engineering; Synthetic Biology
From: Chinese Journal of Biotechnology 2021;37(3):874-910
CountryChina
Language:Chinese
Abstract: The development and implement of microbial chassis cells can provide excellent cell factories for diverse industrial applications, which help achieve the goal of environmental protection and sustainable bioeconomy. The synthetic biology strategy of Design-Build-Test-Learn (DBTL) plays a crucial role on rational and/or semi-rational construction or modification of chassis cells to achieve the goals of "Building to Understand" and "Building for Applications". In this review, we briefly comment on the technical development of the DBTL cycle and the research progress of a few model microorganisms. We mainly focuse on non-model bacterial cell factories with potential industrial applications, which possess unique physiological and biochemical characteristics, capabilities of utilizing one-carbon compounds or of producing platform compounds efficiently. We also propose strategies for the efficient and effective construction and application of synthetic microbial cell factories securely in the synthetic biology era, which are to discover and integrate the advantages of model and non-model industrial microorganisms, to develop and deploy intelligent automated equipment for cost-effective high-throughput screening and characterization of chassis cells as well as big-data platforms for storing, retrieving, analyzing, simulating, integrating, and visualizing omics datasets at both molecular and phenotypic levels, so that we can build both high-quality digital cell models and optimized chassis cells to guide the rational design and construction of microbial cell factories for diverse industrial applications.