Methodological study on eliminating nucleic acid contamination in molecular diagnostic laboratory.

Yunlong LI; Jian Zhang; Yanqiu Wei; Xiaojuan Jia; Xiaoyan LI; Liming Tan; Wenjun Liu; Limin Yang

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Methodological study on eliminating nucleic acid contamination in molecular diagnostic laboratory.

Author: Yunlong LI ¹ ; Jian ZHANG ² ; Yanqiu WEI ¹ ; Xiaojuan JIA ³ ; Xiaoyan LI ⁴ ; Liming TAN ⁵ ; Wenjun LIU ¹ ; Limin YANG ¹
Author Information

1. Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
2. The Third Hospital of Qinhuangdao, Qinhuangdao 066000, Hebei, China.
3. The Biological Safety level-3 (BSL-3) Laboratory of Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
4. The Fifth Affiliated Hospital, Southern Medical University, Guangzhou 510900, Guangdong, China.
5. Jiangxi Province Key Laboratory of Laboratory Medicine, Department of Clinical Laboratory Science, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi, China.
Publication Type:Journal Article
Keywords: PCR; aerosol; nucleic acid contamination; nucleic acid detection
MeSH: Laboratories; Nucleic Acids; Pathology, Molecular
From: Chinese Journal of Biotechnology 2021;37(2):673-679
CountryChina
Language:Chinese
Abstract: Nucleic acid detection technique has good sensitivity and specificity and is widely used in in vitro diagnosis, animal and plant commodity quarantine, forensic identification, and other fields. However, it is susceptible to carryover contamination during the operation and leads to false-positive results, which seriously affects the detection accuracy. Therefore, finding an effective solution to prevent and eliminate nucleic acid carryover contamination has become particularly urgent. This study compared several different methods for removing nucleic acid contamination and confirmed that sodium hypochlorite solution and PCRguard reagent could effectively eliminate nucleic acid carryover in the liquid and on surfaces of different materials. Besides, the combination of sodium hypochlorite solution and PCRguard can solve the nucleic acid aerosol contamination. This study proposes solutions for the routine prevention of carryover contamination and removal of aerosol that has occurred in molecular diagnostic laboratories.