Comparison of urine proteome in rats intraperitoneally infected by different microorganisms.

Wenshu Meng; Yameng Zhang; Yuanrui Hua; Yijin Jiang; Jiawen Kong; Youhe Gao

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Comparison of urine proteome in rats intraperitoneally infected by different microorganisms.

Author: Wenshu MENG ¹ ; Yameng ZHANG ¹ ; Yuanrui HUA ¹ ; Yijin JIANG ¹ ; Jiawen KONG ¹ ; Youhe GAO ¹
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1. Gene Engineering Drug and Biotechnology Beijing Key Laboratory, Department of Biochemistry and Molecular Biology, Beijing Normal University, Beijing 100875, China.
Publication Type:Journal Article
Keywords: differential diagnosis; intraperitoneal infection; proteome; urine
MeSH: Animals; Chromatography, Liquid; Escherichia coli; Proteome; Rats; Staphylococcus aureus; Tandem Mass Spectrometry
From: Chinese Journal of Biotechnology 2021;37(1):276-289
CountryChina
Language:Chinese
Abstract: Different microorganisms can cause intraperitoneal infection. This study was to distinguish different microbial infections by urine analysis. Rats were intraperitoneally injected with Escherichia coli, Staphylococcus aureus, and Candida albicans, separately. Urine samples were collected from rats at 0, 12, 36 and 72 h after infection. Urinary proteins were profiled using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). Compared with the control (without infection), a total of 69 differential proteins were identified in rats injected with E. coli. A total of 31 differences proteins were identified in rats injected with S. aureus. A total of 38 differential proteins were identified in rats injected with C. albicans. Urine proteome was different when rats were infected by different microorganisms, suggesting that urine may have the potential for differential diagnosis of different intraperitoneal infections.