Analysis of differential genes and metabolic pathway related to functional male sterility in eggplant.

Zhimin Wang; Chao Yuan; Zeqin Ding; Ruolin HU; Yi Niu; Qinglin Tang; Dayong Wei; Ming Song; Yongqing Wang; Shibing Tian

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Analysis of differential genes and metabolic pathway related to functional male sterility in eggplant.

Author: Zhimin WANG ¹ ; Chao YUAN ¹ ; Zeqin DING ¹ ; Ruolin HU ¹ ; Yi NIU ¹ ; Qinglin TANG ¹ ; Dayong WEI ¹ ; Ming SONG ¹ ; Yongqing WANG ² ; Shibing TIAN ²
Author Information

1. Key Laboratory of Horticulture Science for Southern Mountainous Regions Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China.
2. The Institute of Vegetables and Flowers, Chongqing Academy of Agricultural Sciences, Chongqing 400055, China.
Publication Type:Journal Article
Keywords: Solanum melongena L.; anther dehiscence; metabolic pathway; transcriptome analysis
MeSH: Flowers/genetics*; Gene Expression Profiling; Gene Expression Regulation, Plant; Humans; Infertility, Male; Male; Metabolic Networks and Pathways/genetics*; Solanum melongena/genetics*; Transcriptome/genetics*
From: Chinese Journal of Biotechnology 2021;37(1):253-265
CountryChina
Language:Chinese
Abstract: Based on observing the cytological characteristics of the flower buds of the functional male sterile line (S13) and the fertile line (F142) in eggplant, it was found that the disintegration period of the annular cell clusters in S13 anther was 2 days later than that of F142, and the cells of stomiun tissue and tapetum in F142 disintegrated on the blooming day, while it did not happen in S13. The comparative transcriptomic analysis showed that there were 1 436 differential expression genes (DEGs) (651 up-regulated and 785 down-regulated) in anthers of F142 and S13 at 8, 5 days before flowering and flowering day. The significance analysis of GO enrichment indicated that there were more unigene clusters involved in single cell biological process, metabolism process and cell process, and more catalytic activity and binding function were involved in molecular functions. Through KEGG annotation we found that the common DEGs were mainly enriched in the biosynthesis of secondary metabolites, metabolic pathway, protein processing in endoplasmic reticulum, biosynthesis of amino acids, carbon metabolism and plant hormone signal transduction. The fifteen genes co-expression modules were identified from 16 465 selected genes by weighted gene co-expression network analysis (WGCNA), three of which (Plum2, Royalblue and Bisque4 modules) were highly related to S13 during flower development. KEGG enrichment showed that the specific modules could be enriched in phenylpropanoid biosynthesis, photosynthesis, porphyrin and chlorophyll metabolism, α-linolenic acid metabolism, polysaccharide biosynthesis and metabolism, fatty acid degradation and the mutual transformation of pentose and glucuronic acid. These genes might play important roles during flower development of S13. It provided a reference for further study on the mechanism of anther dehiscence in eggplant.