Comparison of the antigenicity of African swine fever virus p35 protein as diagnostic antigen.

Lei Shi; Zhancheng Tian; Jifei Yang; Shandian Gao; Junzheng DU; Yaru Zhao; Zhijie Liu; Guiquan Guan; Guangyuan Liu; Jianxun Luo; Hong Yin

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Comparison of the antigenicity of African swine fever virus p35 protein as diagnostic antigen.

Author: Lei SHI ¹ ; Zhancheng TIAN ¹ ; Jifei YANG ¹ ; Shandian GAO ¹ ; Junzheng DU ¹ ; Yaru ZHAO ¹ ; Zhijie LIU ¹ ; Guiquan GUAN ¹ ; Guangyuan LIU ¹ ; Jianxun LUO ¹ ; Hong YIN ¹
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1. State Key Laboratory of Veterinary Etiological Biology/Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, Gansu, China.
Publication Type:Journal Article
Keywords: African swine fever virus; antigenicity; indirect ELISA; p30; p35
MeSH: African Swine Fever/diagnosis*; African Swine Fever Virus/genetics*; Animals; Antibodies, Viral; Enzyme-Linked Immunosorbent Assay; Recombinant Proteins/genetics*; Swine
From: Chinese Journal of Biotechnology 2021;37(1):187-195
CountryChina
Language:Chinese
Abstract: In order to screen African swine fever virus (ASFV) diagnostic antigen with the best enzyme linked immunosorbent assay (ELISA) reactivity. By establishing the ELISA method, the diagnostic antigen of ASFV p30 protein expressed by baculovirus-insect cell expression system as reference, we explored the antigenic properties and diagnostic potential of ASFV p35 protein expressed by prokaryotic expression system as a diagnostic antigen. The results of Western blotting and immunofluorescence show that the molecular weight of the recombinant p35 protein and p30 protein obtained was 40 kDa and 30 kDa, respectively, and these two proteins had good immuno-reactivity with ASFV positive serum. Recombinant p30 and p35 proteins were used as diagnostic antigens to establish ELISA, and the sensitivity and repeatability of these methods were tested. The results show that although the detection sensitivity of the p30-ELISA established in this study was higher than that of the p35-ELISA, the sensitivity of p35-ELISA was 95.8%, and variations in intra- and inter-assay repeatability of the two methods were less than 10%. The coincidence rate between the p35-ELISA and the imported kit was 97.2%. Results show that p35-ELISA was sensitive and stable, and could detect specific antibodies against ASFV.