Progress in the construction and screening of random mutation library.

Author: Jue CHEN ¹ ; Jiamin HUANG ¹ ; Tianhe YAN ¹ ; Xiaoyu PENG ¹ ; Jun LIN ¹
Author Information

1. Institute of Applied Genomics, Fuzhou University, Fuzhou 350108, Fujian, China.
Publication Type:Review
Keywords: directed evolution; high throughput screening; mutant library; random mutation; semi-rational design
MeSH: Base Sequence; Directed Molecular Evolution; Gene Library; Mutation; Proteins/genetics*
From: Chinese Journal of Biotechnology 2021;37(1):163-177
CountryChina
Language:Chinese
Abstract: Directed evolution is a cyclic process that alternates between constructing different genes and screening functional gene variants. It has been widely used in optimization and analysis of DNA sequence, gene function and protein structure. It includes random gene libraries construction, gene expression in suitable hosts and mutant libraries screening. The key to construct gene library is the storage capacity and mutation diversity, to screen is high sensitivity and high throughput. This review discusses the latest advances in directed evolution. These new technologies greatly accelerate and simplify the traditional directional evolution process and promote the development of directed evolution.