Advances in adaptive laboratory evolutionary engineering to microbial breeding.

Jian LI; Jing Kong; Shenglong LI; Yu Zhao; Yakun Zhao; Dongguang Xiao; Aiqun YU

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Advances in adaptive laboratory evolutionary engineering to microbial breeding.

Author: Jian LI ¹ ; Jing KONG ¹ ; Shenglong LI ¹ ; Yu ZHAO ¹ ; Yakun ZHAO ¹ ; Dongguang XIAO ¹ ; Aiqun YU ¹
Author Information

1. Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education, State Key Laboratory of Food Nutrition and Safety, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.
Publication Type:Review
Keywords: adaptive laboratory evolution; adaptive modification; growth rate optimization; long-term adaptation; short-term adaptation; tolerance
MeSH: High-Throughput Nucleotide Sequencing; Laboratories; Mutation
From: Chinese Journal of Biotechnology 2021;37(1):130-141
CountryChina
Language:Chinese
Abstract: In recent years, adaptive laboratory evolution (ALE) has emerged as a powerful tool for basic research in microbiology (e.g., molecular mechanisms of microbial evolution) and efforts on evolutionary engineering of microbial strains (e.g., accelerated evolution of industrial strains by bringing beneficial mutations). The ongoing rapid development of next-generation sequencing platforms has provided novel insights into growth kinetics and metabolism of microbes, and thus led to great advances of this technique. In this review, we summarize recent advances in the applications of long-term and short-term ALE techniques mainly for microbial strain engineering, and different modes of ALE are also introduced. Furthermore, we discuss the current limitations of ALE and potential solutions. We believe that the information reviewed here will make a significant contribution to further advancement of ALE.