Application of sucrose phosphorylase in glycosylation.

Ruini Jiang; Kang YE; Tian Fan; Yuele LU; Linjiang Zhu; Xiaolong Chen; Hanchi Chen

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Application of sucrose phosphorylase in glycosylation.

Author: Ruini JIANG ¹ ; Kang YE ¹ ; Tian FAN ¹ ; Yuele LU ¹ ; Linjiang ZHU ¹ ; Xiaolong CHEN ¹ ; Hanchi CHEN ¹
Author Information

1. College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, China.
Publication Type:Review
Keywords: carbohydrate-active enzymes; enzymatic redesign; glycosylation; structural modification; sucrose phosphorylase
MeSH: Glucosyltransferases/metabolism*; Glycoside Hydrolases/metabolism*; Glycosylation; Glycosyltransferases/genetics*
From: Chinese Journal of Biotechnology 2021;37(1):112-129
CountryChina
Language:Chinese
Abstract: Water solubility, stability, and bioavailability, can be substantially improved after glycosylation. Glycosylation of bioactive compounds catalyzed by glycoside hydrolases (GHs) and glycosyltransferases (GTs) has become a research hotspot. Thanks to their rich sources and use of cheap glycosyl donors, GHs are advantageous in terms of scaled catalysis compared to GTs. Among GHs, sucrose phosphorylase has attracted extensive attentions in chemical engineering due to its prominent glycosylation activity as well as its acceptor promiscuity. This paper reviews the structure, catalytic characteristics, and directional redesign of sucrose phosphorylase. Meanwhile, glycosylation of diverse chemicals with sucrose phosphorylase and its coupling applications with other biocatalysts are summarized. Future research directions were also discussed based on the current research progress combined with our working experience.