Prokaryotic expression, purification and characterization of tissue inhibitor of metalloproteinase-2.

Aiying Xue; Guoxing Feng; Changchun Zhu; Saijun Fan

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Prokaryotic expression, purification and characterization of tissue inhibitor of metalloproteinase-2.

Author: Aiying XUE ¹ ; Guoxing FENG ¹ ; Changchun ZHU ¹ ; Saijun FAN ¹
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1. Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin 300192, China.
Publication Type:Journal Article
Keywords: inclusion body; matrix metalloproteinase; purification; recombinant expression vector; tissue inhibitor of metalloproteinase-2
MeSH: Cloning, Molecular; Escherichia coli/genetics*; Humans; Recombinant Fusion Proteins/genetics*; Recombinant Proteins; Tissue Inhibitor of Metalloproteinase-2/genetics*
From: Chinese Journal of Biotechnology 2020;36(12):2868-2876
CountryChina
Language:Chinese
Abstract: Tissue inhibitor of metalloproteinases-2 (TIMP-2) inhibits tumor migration and invasion. Obtaining TIMP-2 protein is conducive to a comprehensive and in-depth study of its function and mechanism in tumorigenesis and development. We collected human TIMP-2 protein through prokaryotic expression in vitro. We expressed, purified and characterized human TIMP-2 protein. First, the human TIMP-2 gene was cloned from the cDNA obtained by reverse transcription of total RNA of human lung cancer A549 cells, and constructed to pET28a vector. The recombinant plasmid pET28a-TIMP-2 was transformed into Escherichia coli BL21(DE3) after restriction endonuclease digestion and sequencing analysis. The expression of TIMP-2 protein was induced by isopropyl-β-D-thiogalactoside (IPTG), and the expression conditions were optimized. After purification by nickel affinity column, the fusion protein His-TIMP-2 was identified by Western blotting method and its biological activity was detected by gelatin zymography. The fusion protein His-TIMP-2 existed in the form of inclusion body in E. coli. In a certain range, the concentration of IPTG had no significant effect on the expression amount of His-TIMP-2. But in this expression system, induction temperature and time were the key parameters, and the expression amount of His-TIMP-2 in E. coli increased with the increase of induction temperature. The purified and refolded fusion protein could effectively inhibit the activity of matrix metalloproteinases expressed by human lung cancer A549 cells. The acquisition of active fusion protein lays a foundation for further study of the function and mechanism of human TIMP-2, and is of great significance for tumor therapy.