Genome sequencing of Streptomyces aureofaciens DM-1 and analysis of 6-demethylchlortetracycline biosynthesis gene cluster.

Naxin WU; He Huang; Taoling Min; Haifeng HU

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Genome sequencing of Streptomyces aureofaciens DM-1 and analysis of 6-demethylchlortetracycline biosynthesis gene cluster.

Author: Naxin WU ¹ ; He HUANG ² ; Taoling MIN ¹ ; Haifeng HU ¹
Author Information

1. Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, Shanghai 201203, China.
2. Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
Publication Type:Journal Article
Keywords: 6-demethylchlortetracycline; Streptomyces aureofaciens DM-1; biosynthetic gene cluster; genome sequencing
MeSH: Base Sequence; Chlortetracycline; Demeclocycline; Multigene Family/genetics*; Streptomyces aureofaciens/genetics*
From: Chinese Journal of Biotechnology 2020;36(12):2685-2694
CountryChina
Language:Chinese
Abstract: Streptomyces aureofaciens DM-1 is a high-yielding 6-demethylchlortetracycline producer. The genome sequencing of DM-1 reveals a linear chromosome containing 6 824 334 bps nucleotides with GC content of 72.6%. In this genome, a total of 6 431 open reading frames were predicted by using glimmer 3.02, Genemark and Z-Curve softwares. Twenty-eight secondary metabolite biosynthetic gene clusters were uncovered by using AntiSMASH gene prediction software, including the complete 6-demethylchlortetracycline biosynthetic gene cluster. A frame-shift mutation in methyltransferase coding region was detected, which may result in the demethylation of chlortetracycline. The complete genome sequence of S. aureofaciens DM-1 provides basic information for functional genomics studies and selection of high-yielding strains for 6-demethylchlortetracycline.