Effects of Comamonas testosteroni on PAHs degradation and bacterial community structure in Leymus chinensis rhizosphere soil.

Qiao Wang; Rui Zheng; Xueting Sun; Ziwei Jiang; Fanghui Yang; Qian LU; Jizhe Cui

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Effects of Comamonas testosteroni on PAHs degradation and bacterial community structure in Leymus chinensis rhizosphere soil.

Author: Qiao WANG ¹ ; Rui ZHENG ¹ ; Xueting SUN ¹ ; Ziwei JIANG ¹ ; Fanghui YANG ¹ ; Qian LU ¹ ; Jizhe CUI ¹
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1. College of Life Science and Technology, Harbin Normal University, Harbin 150025, Heilongjiang, China.
Publication Type:Journal Article
Keywords: Comamonas testosteroni; Leymus chinensis; bacterial community structure; polycyclic aromatic hydrocarbons; rhizosphere soil
MeSH: Biodegradation, Environmental; Comamonas testosteroni/genetics*; Polycyclic Aromatic Hydrocarbons/analysis*; Rhizosphere; Soil; Soil Microbiology; Soil Pollutants
From: Chinese Journal of Biotechnology 2020;36(12):2657-2673
CountryChina
Language:Chinese
Abstract: To investigate the degradation of polycyclic aromatic hydrocarbons (PAHs) and the changes of rhizosphere microorganisms in the rhizosphere soil of Leymus chinensis during the remediation of PAHs contaminated soil by Comamonas testosteroni (C.t)-assisted Leymus chinensis, we evaluated the removal of PAHs in the rhizosphere of Leymus chinensis using gas chromatography-mass spectrometry (GC-MS), analyzed the bacterial community and the diversity in Leymus chinensis rhizosphere soil by high-throughput sequencing technology, characterized the correlation among PAHs degradation and bacterial community components performing redundancy analysis (RDA) and network analysis, and predicted PAHs degradation potential via PICRUSt software in this paper. The degradation of PAHs in the rhizosphere of Leymus chinensis was promoted, the abundance and diversity of bacteria and the correlation among bacteria and PAHs were changed, and the degradation potential of PAHs in Leymus chinensis rhizosphere soil was enhanced in the later stage of phytoremediation (60-120 d) due to the incorporation of C.t. The accelerated degradation of three PAHs (Nap, Phe, BaP) was accompanied by the differ abundance and correlation of Proteobacteria (Sphingomonas, MND1, Nordella), Actinomycetes (Rubrobacter, Gaiella), Acidobacteria (RB41) and Bacteroides (Flavobacterium) affected by C.t. The results provide new insight into the microorganism choices for microbial assisted plant remediation of soil PAHs and the mechanisms of enhanced PAHs degradation via the combination of Comamonas testosteroni engineering bacteria and plants.