High-throughput sequencing analysis of intestinal flora diversity of two freshwater snails (Radix auricularia and Planorbella trivolvis).

Zongfu HU; Jie Chang; Qing Tong; Jianhua YU; Shuguo LI; Huaxin Niu

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High-throughput sequencing analysis of intestinal flora diversity of two freshwater snails (Radix auricularia and Planorbella trivolvis).

Author: Zongfu HU ¹ ; Jie CHANG ¹ ; Qing TONG ² ; Jianhua YU ¹ ; Shuguo LI ¹ ; Huaxin NIU ¹
Author Information

1. College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao 028000, Inner Mongolia, China.
2. College of Veterinary Medicine, Northeast Agricultural University, Harbin 150038, Heilongjiang, China.
Publication Type:Journal Article
Keywords: 16S rRNA high-throughput sequencing; Planorbella trivolvis; Radix auricularia; gut microbiota
MeSH: Animals; Auricularia; Ecosystem; Fresh Water; Gastrointestinal Microbiome/genetics*; High-Throughput Nucleotide Sequencing; Phylogeny; RNA, Ribosomal, 16S/genetics*; Snails
From: Chinese Journal of Biotechnology 2020;36(12):2622-2634
CountryChina
Language:Chinese
Abstract: Freshwater snail is an important biological group in aquatic ecosystem and an intermediate host of many parasites. Intestinal flora plays an important role in animal energy metabolism and resistance to pathogens. We analyzed the intestinal microbiota diversity of Radix auricularia (RA) and Planorbella trivolvis (PL) by 16S rRNA high-throughput sequencing. At the phylum level, RA had 23 phyla, including Proteobacteria (33.63%), Cyanobacteria (15.33%), Chloroflexi (13.95%), and Actinomycetes (12.99%). PL had 13 phyla, including Proteobacteria (54.88%), Bacteroidetes (28.49%), and Actinomycetes (7.65%). At the genus level, there were 445 genera in RA, including Pleurocapsa, Thiodictyon, Leptotrichia, and Nocardioides. There were 238 genera in PL, including Cloacibacterium, OM60NOR5_clade, Pseudomonas, and Rhodobacter. Ninety-three genera were the common core flora of the two snail species (all the samples were present), and 27 genera had an abundance greater than 0.5%. The structure of intestinal microbiota was significantly different between the two groups (P=0.027). We performed the functional prediction of intestinal microbiota using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt), and the results show that the KEGG functional composition of the intestinal flora of the two snails was similar, and the abundance of the amino acid metabolism, carbohydrate metabolism and membrane transport were large. In summary, the intestinal microbiota of the two snails was high in diversity and significantly different, but there were a large number of common core flora.