Effects of substrate on growth and lipid accumulation of Tribonema sp. FACHB-1786.

Ting Zhang; Qing HE; Zijun XU; Feiya Suo; Chengwu Zhang; Qiang HU

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Effects of substrate on growth and lipid accumulation of Tribonema sp. FACHB-1786.

Author: Ting ZHANG ¹ ; Qing HE ² ; Zijun XU ³ ; Feiya SUO ¹ ; Chengwu ZHANG ³ ; Qiang HU ²
Author Information

1. College of Life Science and Technology, Xinjiang University, Urumchi 830046, Xinjiang, China.
2. Center for Microalgal Biotechnology and Biofuels, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, Hubei, China.
3. Research Center of Hydrobiology, Department of Ecology, Jinan University, Guangzhou 510632, Guangdong, China.
Publication Type:Journal Article
Keywords: Tribonema sp.; algal oil; eicosapentaenoic acid; microalgal culture; mineral nutrients; palmitoleic acid
MeSH: Biofuels; Biomass; Lipids; Microalgae; Nitrogen; Stramenopiles
From: Chinese Journal of Biotechnology 2020;36(11):2478-2493
CountryChina
Language:Chinese
Abstract: Filamentous microalga Tribonema sp. has the advantages of highly resistance to zooplankton-predation, easy harvesting, and high cellular lipid content, in particular large amounts of palmitoleic acid (PA) and eicosapentaenoic acid (EPA). Therefore, Tribonema sp. is considered as a promising biomass feedstock to produce biodiesel and high-value products. In this work, we studied the effect of different concentrations of nitrogen (NaNO₃: 255-3 060 mg/L), phosphorus (K₂HPO₄: 4-240 mg/L), iron ((NH₄)₃FeC₁₂H₁₀O₁₄: 0.6-12 mg/L) and magnesium (MgSO₄: 7.5-450 mg/L) on the biomass, lipid content, and fatty acid composition of Tribonema sp. FACHB-1786, aiming at enhancing cell lipid productivity. The growth of Tribonema sp. had a positive correlation with the concentration of magnesium, and the maximum biomass of Tribonema sp. (under the condition of 450 mg/L MgSO₄) was 8.09 g/L, much greater than those reported in previous studies using the same and other Tribonema species under autotrophic conditions. Different nitrogen concentrations exerted no significant effect on algal growth (P > 0.05), but a higher nitrogen concentration resulted in a greater amount of lipid in the cells. The maximum volumetric productivities of total lipids (319. 6 mg/(L·d)), palmitoleic acid (135.7 mg/(L·d)), and eicosapentaenoic acid (24.2 mg/(L·d)) of Tribonema sp. were obtained when the concentrations of NaNO₃, K₂HPO₄, (NH₄)₃FeC₁₂H₁₀O₁₄, and MgSO₄ were 765 mg/L, 80 mg/L, 6 mg/L, and 75 mg/L, respectively. This study will provide a reference for substrate optimization for Tribonema sp. growth and lipid production.