Application of glycan microarrays in cancer research.

Author: Beining XU ¹ ; Yinkun LIU ² ; Yu'an XIE ¹
Author Information

1. Department of Oncology Research, Guangxi Medical University Affiliated Tumor Hospital & Oncology Medical College, Nanning 530021, Guangxi, China.
2. Key Laboratory of Carcinogenesis and Cancer Invasion (Ministry of Education), Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Publication Type:Journal Article
Keywords: cancer vaccines; diagnostic biomarkers; glycan binding proteins; glycan microarray; monoclonal antibody
MeSH: Antibodies, Monoclonal; Glycosylation; Lectins/metabolism*; Microarray Analysis; Neoplasms; Polysaccharides
From: Chinese Journal of Biotechnology 2020;36(11):2313-2326
CountryChina
Language:Chinese
Abstract: Glycosylation is one of the common post-translational modifications of proteins to regulate the ability of tumor invasion, metastasis and tumor heterogeneity by interacting with glycan-binding proteins such as lectins and antibodies. Glycan microarray can be constructed by chemical synthesis, chemical-enzyme synthesis or natural glycan releasing. Glycan microarray is an essential analytical tool to discover the interaction between glycan and its binding proteins. Here we summarize the standard techniques to construct glycan microarray for the application in cancer vaccine, monoclonal antibody and diagnostic markers.