Light and carbon dioxide-driven synthesis of high-density fuel in Synechococcus elongates UTEX 2973.
- Author:
Shubin LI
1
;
Tao SUN
1
;
Lei CHEN
1
;
Weiwen ZHANG
1
Author Information
- Publication Type:Journal Article
- Keywords: Synechococcus elongates UTEX 2973; high-density cultivation; high-density fuel; liquid sunshine; β-caryophyllene
- MeSH: Biofuels/microbiology*; Carbon Dioxide/metabolism*; Light; Photosynthesis; Synechococcus/radiation effects*
- From: Chinese Journal of Biotechnology 2020;36(10):2126-2138
- CountryChina
- Language:Chinese
- Abstract: Development of "liquid sunshine" could be a key technology to deal with the issue of fossil fuel depletion. β-caryophyllene is a terpene compound with high energy density and has attracted attention for its potential application as a jet fuel. The high temperature and high light-tolerant photosynthetic cyanobacterium Synechococcus elongatus UTEX 2973 (hereafter Synechococcus 2973), whose doubling time is as short as 1.5 h, has great potential for synthesizing β-caryophyllene using sunlight and CO₂. In this study, a production of ~121.22 μg/L β-caryophyllene was achieved at 96 h via a combined strategy of pathway construction, key enzyme optimization and precursor supply enhancement. In addition, a final production of ~212.37 μg/L at 96 h was realized in a high-density cultivation. To our knowledge, this is the highest production reported for β-caryophyllene using cyanobacterial chassis and our study provide important basis for high-density fuel synthesis in cyanobacteria.
