Three-dimensional chromosome conformation capture and its derived technologies.

Author: Hao TIAN ¹ ; Zijian YANG ¹ ; Xingwen XU ¹ ; Liangyu LIU ¹
Author Information

1. Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, College of Life Sciences, Capital Normal University, Beijing 100048, China.
Publication Type:Review
Keywords: chromatin immunoprecipitation; chromatin interaction analysis by paired-end tag sequencing; chromosome conformation capture; high-throughput chromatin conformation capture; three-dimension genome
MeSH: Cell Nucleus; Chromatin/genetics*; Chromosomes/genetics*; Genetic Techniques; Genome/genetics*
From: Chinese Journal of Biotechnology 2020;36(10):2040-2050
CountryChina
Language:Chinese
Abstract: Linear chromatin is compacted into eukaryotic nucleus through a complex and multi-layered architecture. Consequently, chromatin conformation in a local or long-distance manner is strongly correlated with gene expression. Chromosome conformation capture (3C) technology, together with its variants like 4C/5C/Hi-C, has been well developed to study chromatin looping and whole genome structure. In this review, we introduce new technologies including chromosome capture combined with immunoprecipitation, nuclei acid-based hybridization, single cell and genome sequencing, as well as their application.