Expressions of cytokeratin 14 and proliferating cell nuclear antigen in the Hertwig's epithelial root sheath of a Vps4b knockout mouse.

Qing Tian; Ying-ying Wang; Qiang LI; Dong Chen

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Expressions of cytokeratin 14 and proliferating cell nuclear antigen in the Hertwig's epithelial root sheath of a Vps4b knockout mouse.

Author: Qing TIAN ¹ ; Ying-Ying WANG ² ; Qiang LI ¹ ; Dong CHEN ¹
Author Information

1. Dept. of Stomatology, The First Affiliated Hospital of Zhengzhou University, Henan Provincial Stomatological Hospital, Zhengzhou 450052, China.
2. Dept. of Stomatology, Xuzhou Central Hospital, Xuzhou 221009, China.
Publication Type:Journal Article
Keywords: Hertwig's epithelial root sheath; Vps4b gene; cytokeratin 14; gene knockout; proliferating cell nuclear antigen
MeSH: ATPases Associated with Diverse Cellular Activities; Animals; Endosomal Sorting Complexes Required for Transport; Epithelial Cells; Keratin-14; Mice; Mice, Knockout; Proliferating Cell Nuclear Antigen; Tooth Root
From: West China Journal of Stomatology 2021;39(3):274-278
CountryChina
Language:English
Abstract: OBJECTIVES:The effect of Vps4b gene mutation on the expressions of cytokeratin 14 (CK14) and proliferating cell nuclear antigen (PCNA) in the Hertwig's epithelial root sheath (HERS) is investigated.
METHODS:The bilateral mandibular tissues of mouse on postnatal days 5, 9, 11, 15, and 19 were removed. The mandibular first molar tissue sections were obtained after paraffin embedding. The CK14 and PCNA expressions in the epithelial root sheath of the normal mouse and Vps4b knockout mouse were compared through immunohistochemistry.
RESULTS:On postnatal day 5, the normal mouse began to form HERS and had a strong positive PCNA expression in the HERS cells; on postnatal day 9, the HERS structure was continuous, and PCNA was positive in the HERS cells; on postnatal day 11, a small portion of HERS began to break, and PCNA was weakly positive in the HERS cells; on postnatal day 15, HERS continued to fracture; PCNA was weakly and positively expressed in the HERS cells on the root surface; on postnatal day 19, the tooth root reached normal physiological length, and PCNA was positively expressed in the HERS cells of the terminal part. Similar to the normal mouse, the gene knockout mouse also formed a HERS structure on postnatal day 5. However, HERS began to break on postnatal day 9. On postnatal day 19, only a few fragments of HERS were found on the root surface, and the root development was immature. Moreover, the expression intensity of PCNA in the gene knockout mouse was decreased.
CONCLUSIONS:The Vps4b gene mutation may change the CK14 and PCNA expressions, leading to abnormal root development.