Effect of ionizing radiation on the secretion of the paracellular pathway in rat submandibular glands.
- Author:
Yan-Hui WU
1
;
Hui XU
2
;
Qing-Ting YAO
1
;
Shao-Hua LIU
3
;
Aipiziguli YAKUPU
1
;
Li-Dan LU
1
;
Liang SHI
3
Author Information
- Publication Type:Journal Article
- Keywords: claudin-4; ionizing radiation; paracellular pathway; submandibular gland; tight junction
- MeSH: Animals; Microscopy, Electron, Transmission; Radiation, Ionizing; Rats; Rats, Wistar; Submandibular Gland; Tight Junctions
- From: West China Journal of Stomatology 2021;39(3):267-273
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:This study aims to investigate the effects of ionizing radiation on the secretion of the paracellular pathway in rat submandibular glands (SMGs) and reveal the changes in the tight junction (TJ) protein claudin-4.
METHODS:A total of 24 Wistar rats were randomly divided into control and irradiation groups. The irradiation groups were further divided into 1, 4, and 12 weeks groups after irradiation. One-time 20 Gy irradiation was given to the SMG area on the experimental side of the irradiation group. At 1, 4, and 12 weeks after irradiation, the secretion of SMGs was measured using the Schirmer's test. The pathological changes in the gland tissues were observed under light microscopy after hematoxylin⁃eosin (HE) staining. The changes in the TJ ultrastructure were observed under transmission electron microscopy. The immunofluorescence staining and Western blot were used to detect the expression levels of muscarinic acetylcholine M3 receptor, aquaporin 5 (AQP5), and claudin-4 protein.
RESULTS:At 1, 4, and 12 weeks after irradiation, the secretion of SMGs in the irradiation group was significantly decreased and lower than that in the control group (
CONCLUSIONS:The changes in the TJ structure, the upregulation of the claudin-4 expression, and the damage in the paracellular pathway were involved in the hyposecretion of SMGs after irradiation.
