Protective effect of low-level laser irradiation on lipopolysaccharide-mediated inflammatory injury of human periodontal ligament fibroblasts.

Xiao-lan Jin; Ya-nan Zhang; Cheng-rui Sun; Zhao-hui Zou

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Protective effect of low-level laser irradiation on lipopolysaccharide-mediated inflammatory injury of human periodontal ligament fibroblasts.

Author: Xiao-Lan JIN ¹ ; Ya-Nan ZHANG ² ; Cheng-Rui SUN ¹ ; Zhao-Hui ZOU ³
Author Information

1. Dept. of Stomatology, Tianjin Jinnan Hospital, Tianjin 300350, China.
2. Dept. of Stomatology, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300192, China.
3. Dept. of Endodontics Third Room, School of Stomatology, Tianjin Medical University, Tianjin 300070, China.
Publication Type:Journal Article
Keywords: effect; human periodontal ligament fibroblasts; inflammatory injury; lipopolysaccharide; low-level laser irradiation
MeSH: Cells, Cultured; Fibroblasts; Humans; Interleukin-1beta; Lasers; Lipopolysaccharides; Periodontal Ligament; Tumor Necrosis Factor-alpha
From: West China Journal of Stomatology 2021;39(3):260-266
CountryChina
Language:English
Abstract: OBJECTIVES:To study the effect and mechanism of low-level laser irradiation (LLLI) on lipopolysaccharide (LPS)-induced inflammatory injury of human periodontal ligament fibroblasts (hPDLFs).
METHODS:hPDLFs were inoculated into well plates and randomly divided into the normal group, LPS group, and LPS+LLLI group. The cells in the normal group were cultured in conventional medium. The hPDLFs in the LPS and LPS+LLLI groups were cultured in RPMI1640 medium containing 1 mg·L
RESULTS:Compared with the normal group, the LPS group showed increased apoptosis rate of hPDLFs and intracellular free Ca
CONCLUSIONS:LLLI has a protective effect on the inflammatory injury of hPDLFs induced by LPS, and the effect is most obvious when the irradiation intensity is 4 J·cm