Expression of cyclophilin A in oral squamous cell carcinoma and its effect on cell proliferation and invasion.
- Author:
Xiao-Yang XIA
1
;
Fei FANG
1
;
Yan LIU
1
;
Chao CHE
1
;
Jin-Juan KE
1
;
Sheng-Jun JIANG
2
Author Information
- Publication Type:Journal Article
- Keywords: cell proliferation; cyclophilin A; invasiveness; oral squamous cell carcinoma
- MeSH: Carcinoma, Squamous Cell/genetics*; Cell Line, Tumor; Cell Movement; Cell Proliferation; Cyclophilin A/genetics*; Gene Expression Regulation, Neoplastic; Head and Neck Neoplasms; Humans; Mouth Neoplasms/genetics*; Squamous Cell Carcinoma of Head and Neck
- From: West China Journal of Stomatology 2021;39(2):164-169
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To investigate the expression of cyclophilin A (CyPA) in oral squamous cell carcinoma (OSCC) and explore the effect of downregulating the expression of CyPA gene on the proliferation and invasion of SCC-25 cells.
METHODS:A total of 77 cases of patients with OSCC were selected. The expression levels of CyPA proteins in OSCC and adjacent normal tissues were evaluated. SCC-25 cells were cultured and divided into the CyPA interference sequence group, negative control group, and blank group. The expression levels of CyPA mRNA and protein in cells were detected by using real-time fluorescent quantitative polymerase chain reaction and Western blot, respectively. Cell proliferation was detected by using methyl thiazolyl tetrazolium and plate colony formation assays. Cell invasion was detected by using Transwell assay.
RESULTS:The positive expression rate of CyPA protein in OSCC tissues was 76.62%, which was higher than that in adjacent tissues (
CONCLUSIONS:The CyPA protein is highly expressed in OSCC tissues, and the downregulation of CyPA gene expression in SCC-25 cells can reduce cell proliferation and inhibit cell invasion.
