Effects of isoprenylcysteine carboxyl methyltransferase silencing on the proliferation and apoptosis of tongue squamous cell carcinoma.

Shao-ru Wang; Wei Sun; Nan Zhou; Kai Zhao; Wen-jian LI; Zeng-peng Chi; Ying Wang; Qi-min Wang; Lei Tong; Zong-xuan HE; Hong-yu Han; Zheng-gang Chen

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Effects of isoprenylcysteine carboxyl methyltransferase silencing on the proliferation and apoptosis of tongue squamous cell carcinoma.

Author: Shao-Ru WANG ¹ ; Wei SUN ¹ ; Nan ZHOU ² ; Kai ZHAO ³ ; Wen-Jian LI ⁴ ; Zeng-Peng CHI ² ; Ying WANG ⁵ ; Qi-Min WANG ¹ ; Lei TONG ¹ ; Zong-Xuan HE ⁶ ; Hong-Yu HAN ¹ ; Zheng-Gang CHEN ¹
Author Information

1. Dept. of Stomatology, Qingdao Municipal Hospital, Qingdao University, Qingdao 266071, China.
2. College of Stomatology, Weifang Medical University, Weifang 261021, China.
3. School of Stomatology, Qingdao University, Qingdao 266003, China.
4. School of Stomatology, Dalian Medical University, Dalian 116044, China.
5. Dept. of Stomatology, Fourth People,s Hospital of Jinan, Jinan 250031, China.
6. Dept. of Oral and Maxillafacial Surgery, The Affiliated Hospital of Qingdao University, Qingdao 266005, China.
Publication Type:Journal Article
Keywords: RhoA; apoptosis; cell cycle; cell proli-feration; isoprenylcysteine carboxyl methyltransferase; tongue squamous cell carcinoma
MeSH: Apoptosis; Carcinoma, Squamous Cell; Cell Line, Tumor; Cell Proliferation; Humans; Protein Methyltransferases; RNA, Small Interfering; Tongue; Tongue Neoplasms
From: West China Journal of Stomatology 2021;39(1):64-73
CountryChina
Language:English
Abstract: OBJECTIVES:This study aimed to explore the effects of silencing isoprenylcysteine carboxyl methyltransfe-rase (Icmt) through small interfering RNA (siRNA) interference on the proliferation and apoptosis of tongue squamous cell carcinoma (TSCC).
METHODS:Three siRNA were designed and constructed for the Icmt gene sequence and were then transfected into TSCC cells CAL-27 and SCC-4 to silence Icmt expression. The tested cells were divided as follows: RNA interference groups Icmt-siRNA-1, Icmt-siRNA-2, and Icmt-siRNA-3, negative control group, and blank control group. The transfection efficiency of siRNA was detected by the fluorescent group Cy3-labeled siRNA, and the expression of Icmt mRNA was screened by quantitive real-time polymerase chain reaction (qRT-PCR) selected the experimental group for subsequent experiments. The expression of Icmt, RhoA, Cyclin D1, p21, extracellular regulated protein kinases (ERK), and phospho-extracellular regulated protein kinases (p-ERK) were analyzed by Western blot. The proliferation abilities of TSCC cells were determined by cell counting kit-8 assay. The change in apoptosis was detected by AnnexinV-APC/propidium staining (PI) assay. Cell-cycle analysis was conducted by flow cytometry.
RESULTS:The expression of Icmt mRNA and protein in TSCC cells significantly decreased after Icmt-siRNA transfection (
CONCLUSIONS:Silencing Icmt can effectively downregulate its expression in TSCC cells, reduce the RhoA membrane targeting localization and cell proliferation, and induce apoptosis. Thus, Icmt may be a potential gene therapy target for TSCC.