Effect of

Yi Qun Liu; Ling He Huang; Pei Pei Liu; Qing Bin Xing; Feng Han; Qin Wang; Shu Rong Chen; Kimio Sugiyama; Xue Song Xiang; Zhen Wu Huang

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Effect of

Author: Yi Qun LIU ¹ ; Ling He HUANG ¹ ; Pei Pei LIU ² ; Qing Bin XING ¹ ; Feng HAN ¹ ; Qin WANG ¹ ; Shu Rong CHEN ³ ; Kimio SUGIYAMA ⁴ ; Xue Song XIANG ¹ ; Zhen Wu HUANG ¹
Author Information

1. Key Laboratory of Trace Element Nutrition of National Health Commission, National Institute of Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
2. Chinese Nutrition Society, Beijing 100022, China.
3. Beijing Dietetic Association, Beijing 100050, China.
4. Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.
Publication Type:Journal Article
Keywords: 5-methyltetrahydrofolate; Folate deficiency; N,N-Dimethylglycine; Plasma homocysteine
MeSH: Animals; Biomarkers/metabolism*; Chromatography, Liquid; Diet; Dietary Supplements; Folic Acid Deficiency/metabolism*; Homocysteine/metabolism*; Liver/metabolism*; Male; Random Allocation; Rats; Rats, Wistar; Sarcosine/metabolism*
From: Biomedical and Environmental Sciences 2021;34(5):356-363
CountryChina
Language:English
Abstract: Objective:This study aimed to investigate the effects of
Methods:In this study, 0.1% DMG was supplemented in 20% casein diets that were either folate-sufficient (20C) or folate-deficient (20CFD). Blood and liver of rats were subjected to assays of Hcy and its metabolites. Hcy and its related metabolite concentrations were determined using a liquid chromatographic system.
Results:Folate deprivation significantly increased pHcy concentration in rats fed 20C diet (from 14.19 ± 0.39 μmol/L to 28.49 ± 0.50 μmol/L;
Conclusion:DMG supplementation exhibited hypohomocysteinemic effects under folate-sufficient conditions. By contrast, the combination of folate deficiency and DMG supplementation has deleterious effect on pHcy concentration.