Exploration of an Efficient Simultaneous Molecular Detection Method of HIV, HCV, and Syphilis from a Single Dried Blood Spot.
- Author:
Jie Qiong MA
1
;
Qing Qing XU
1
;
Lin HE
1
;
Xiao Xia HE
1
;
Kai CHEN
1
;
Yue Hua WANG
1
;
Wen Ge XING
1
;
Yan JIANG
1
Author Information
- Publication Type:Journal Article
- Keywords: Bland-Altman; Correlation; Dried blood spot (DBS); HCV RNA; HIV-1 DNA; HIV-1 RNA
- MeSH: DNA, Viral/analysis*; Diagnostic Tests, Routine/methods*; Dried Blood Spot Testing/methods*; HIV Infections/diagnosis*; HIV-1/isolation & purification*; Hepacivirus/isolation & purification*; Hepatitis C/diagnosis*; RNA, Viral/analysis*; Sensitivity and Specificity; Specimen Handling/methods*; Syphilis/diagnosis*; Treponema pallidum/isolation & purification*
- From: Biomedical and Environmental Sciences 2021;34(4):257-264
- CountryChina
- Language:English
-
Abstract:
Objective:The aim of the present study was to evaluate the performance of the simultaneous detection of HIV-1 RNA, HIV-1 DNA, and HCV RNA using one dried blood spot (DBS) as an alternative sample to plasma.
Method:A total of 571 paired DBS/plasma samples were collected from men who have sex with men (MSM) and injection drug users (IDUs), and serological and molecular assays were performed. Using plasma results as the reference standard, the performance of DBS tests for HIV-1 RNA, HIV-1 DNA, and HCV RNA was evaluated. Pearson's correlation coefficients and Bland-Altman analysis were performed to assess the correlation and concordance between DBS and plasma.
Results:Among paired plasma/DBS samples with detectable HIV-1 RNA and HCV RNA, five samples (5/32) were not detectable in DBS, while measurable HIV-1 RNA levels were present in plasma (1.44 to 3.99 log
Conclusion:The performance of the simultaneous detection of HIV-1 RNA, HIV-1 DNA, and HCV RNA using one DBS was acceptable. DBS, as an alternative sample to plasma, may be a viable option for the simultaneous detection of HIV-1 RNA, HIV-1 DNA, and HCV RNA in resource-limited settings or for individuals living in areas that are difficult to access.
