RT-nPCR Assays for Amplification and Sequencing of VP1 Genes in Human Enterovirus A-D from Clinical Specimens.
- Author:
Wei CHEN
1
;
Yu Wei WENG
2
;
Wen Xiang HE
2
;
Ying ZHU
2
;
Ting Ting YU
2
;
Jian Feng XIE
2
;
Kui Cheng ZHENG
2
;
Yan Sheng YAN
2
;
Yong Jun ZHANG
2
;
Wen Chang ZHANG
1
Author Information
- Publication Type:Journal Article
- Keywords: Clinical specimens; Human enterovirus A–D; Polymerase chain reaction; VP1 gene
- MeSH: Capsid Proteins/genetics*; Enterovirus A, Human/genetics*; Enterovirus B, Human/genetics*; Enterovirus C, Human/genetics*; Enterovirus D, Human/genetics*; Humans; Molecular Epidemiology/methods*; Molecular Typing/methods*; Reverse Transcriptase Polymerase Chain Reaction/methods*
- From: Biomedical and Environmental Sciences 2020;33(11):829-838
- CountryChina
- Language:English
-
Abstract:
Objective:To develop RT-nPCR assays for amplifying partial and complete VP1 genes of human enteroviruses (HEVs) from clinical samples and to contribute to etiological surveillance of HEV-related diseases.
Methods:A panel of RT-nPCR assays, consisting of published combined primer pairs for VP1 genes of HEV A-C and in-house designed primers for HEV-D, was established in this study. The sensitivity of each RT-nPCR assay was evaluated with serially diluted virus stocks of five serotypes expressed as CCID
Results:The sensitivity of RT-nPCR assays for amplifying partial VP1 gene of HEVs was 0.1 CCID
Conclusion:This RT-nPCR system is capable of amplifying the partial and complete VP1 gene of HEV A-D, providing rapid, sensitive, and reliable options for molecular typing and molecular epidemiology of HEVs in clinical specimens.
