Effects of Toluene on the Development of the Inner Ear and Lateral Line Sensory System of Zebrafish.
- Author:
Xu Dong LI
1
,
2
;
Hong Wei TU
3
;
Ke Qi HU
4
;
Yun Gang LIU
4
;
Li Na MAO
3
;
Feng Yan WANG
3
;
Hong Ying QU
5
,
6
;
Qing CHEN
7
Author Information
- Publication Type:Journal Article
- Keywords: Lateral line sensory system; Ototoxicity; Toluene; Zebrafish
- MeSH: Animals; Ear, Inner/growth & development*; Embryo, Nonmammalian/drug effects*; Gene Expression Regulation, Developmental/drug effects*; Hair Cells, Auditory/metabolism*; Lateral Line System/growth & development*; Locomotion/drug effects*; Ototoxicity/physiopathology*; Toluene/toxicity*; Zebrafish
- From: Biomedical and Environmental Sciences 2021;34(2):110-118
- CountryChina
- Language:English
-
Abstract:
Objective:The aim of this study was to explore the ototoxicity of toluene in the early development of zebrafish embryos/larvae.
Methods:Zebrafish were utilized to explore the ototoxicity of toluene. Locomotion analysis, immunofluorescence, and qPCR were used to understand the phenotypes and molecular mechanisms of toluene ototoxicity.
Results:The results demonstrated that at 2 mmol/L, toluene induced zebrafish larvae death at 120 hours post fertilization (hpf) at a rate of 25.79% and inhibited the rate of hatching at 72 hpf. Furthermore, toluene exposure inhibited the distance travelled and average swimming velocity of zebrafish larvae while increasing the frequency of movements. As shown by fluorescence staining of hair cells, toluene inhibited the formation of lateral line neuromasts and middle line 1 (Ml
Conclusion:This study indicated that toluene may affect the development of both the inner ear and lateral line systems in zebrafish, while the lateral line system may be more sensitive to toluene than the inner ear.
