MicroRNA-135a in ABCA1-labeled Exosome is a Serum Biomarker Candidate for Alzheimer's Disease.

Chen Geng Liu; Shuang Meng; Ying LI; Yao LU; Yue Zhao; Pei Chang Wang

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MicroRNA-135a in ABCA1-labeled Exosome is a Serum Biomarker Candidate for Alzheimer's Disease.

Author: Chen Geng LIU ¹ ; Shuang MENG ² ; Ying LI ³ ; Yao LU ¹ ; Yue ZHAO ¹ ; Pei Chang WANG ¹
Author Information

1. Clinical Laboratory of Xuanwu Hospital, Captital Medcial University, Beijing 100053, China.
2. State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Beijing 102206, China.
3. Clinical Laboratory of Air Force General Hospital, Chinese People's Liberation Army, Beijing 100142, China.
Publication Type:Journal Article
Keywords: Alzheimer’s disease; Biomarker; Exosome; MicroRNA
MeSH: ATP Binding Cassette Transporter 1/cerebrospinal fluid*; Aged; Aged, 80 and over; Alzheimer Disease/cerebrospinal fluid*; Animals; Biomarkers/cerebrospinal fluid*; Cell Line; Cognitive Dysfunction/cerebrospinal fluid*; Erythrocytes/metabolism*; Exosomes; Female; Humans; Leukocytes/metabolism*; Male; Mice, Transgenic; MicroRNAs/blood*; Neurons/metabolism*
From: Biomedical and Environmental Sciences 2021;34(1):19-28
CountryChina
Language:English
Abstract: Objective:In the present study, the ABCA1 was used as a label to capture specific exosomes, the level of ABCA1-labeled exosomal microRNA-135a (miR-135a) was evaluated for the diagnosis of Alzheimer's disease (AD), especially in patients with early stages of AD.
Methods:This is a preliminary research focused on the levels of ABCA1 in WBCs, RBCs, HT-22 cells, and neuron cells. The diagnostic value of ABCA1-labeled exosomal miR-135a was examined using the CSF and serum of APP/PS1 double transgenic mice, and 152 patients with SCD, 131 patients with MCI, 198 patients with DAT, and 30 control subjects.
Results:The level of ABCA1 exosomes harvested from HT-22 cells and neuron culture medium was significantly higher compared to that of RBCs and WBCs (
Conclusion:This study outlines a method to capture specific exosomes and detect them using immunological methods, which is more efficient for early diagnosis of AD.