Efficacy of Essential Trace Elements Supplementation on Mineral Composition, Sperm Characteristics, Antioxidant Status, and Genotoxicity in Testis of Tebuconazole-treated Rats.

Hajer Ben Saad; Fatma Ben Abdallah; Intidhar Bkhairia; Ons Boudawara; Moncef Nasri; Ahmed Hakim; Ibtissem Ben Amara

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Efficacy of Essential Trace Elements Supplementation on Mineral Composition, Sperm Characteristics, Antioxidant Status, and Genotoxicity in Testis of Tebuconazole-treated Rats.

Author: Hajer BEN SAAD ¹ ; Fatma BEN ABDALLAH ² ; Intidhar BKHAIRIA ¹ ; Ons BOUDAWARA ³ ; Moncef NASRI ¹ ; Ahmed HAKIM ⁴ ; Ibtissem BEN AMARA ¹
Author Information

1. Laboratory of Enzyme engineering and Microbiology, National Engineering School in Sfax, University of Sfax, B.P. 1173, 3038 Sfax, Tunisia.
2. Laboratory of Human Molecular Genetics, Faculty of Medicine of Sfax, University of Sfax, Tunisia.
3. Laboratory of Anatomopathology, CHU Habib Bourguiba, University of Sfax, 3029 Sfax, Tunisia.
4. Laboratory of Pharmacology, Faculty of Medicine, 3029 Sfax, University of Sfax, Tunisia.
Publication Type:Journal Article
Keywords: Copper; Iron; Selenium; Testicular toxicity; Zinc
MeSH: Animal Feed/analysis*; Animals; Antioxidants/metabolism*; Diet; Dietary Supplements/analysis*; Fungicides, Industrial/adverse effects*; Male; Minerals/metabolism*; Mutagenicity Tests; Rats; Rats, Wistar; Spermatozoa/physiology*; Testis/physiology*; Trace Elements/metabolism*; Triazoles/adverse effects*
From: Biomedical and Environmental Sciences 2020;33(10):760-770
CountryChina
Language:English
Abstract: Objective:This research was performed to evaluate the effect of tebuconazole (TBZ) on reproductive organs of male rats and to assess the protective role of combined essential trace elements in alleviating the detrimental effect of TBZ on male reproductive function.
Methods:For this purpose, 48 rats were exposed to 100 mg/kg TBZ, TBZ supplemented with zinc (Zn), selenium (Se), copper (Cu), and iron (Fe), TBZ + (Se + Zn); TBZ + Cu; or TBZ + Fe. The experiment was conducted for 30 consecutive days.
Results:TBZ caused a significant perturbation in mineral levels and reduction in reproductive organs weights, plasma testosterone level, and testicular antioxidant enzyme activities. The TBZ-treated group also showed a significant increase in sperm abnormalities (count, motility, and viability percent), plasma follicle-stimulating hormone and luteinizing hormone concentrations, lipid peroxidation, protein oxidation, and severe DNA degradation in comparison with the controls. Histopathologically, TBZ caused testis impairments. Conversely, treatment with trace elements, in combination or alone, improved the reproductive organ weights, sperm characteristics, TBZ-induced toxicity, and histopathological modifications in testis.
Conclusion:TBZ exerts significant harmful effects on male reproductive system. The concurrent administration of trace elements reduces testis dysfunction, fertility, and toxicity induced by TBZ.