Positive inotropic effect of phosphodiesterase type 9 inhibitor PF-04449613 in rats and its underlying mechanism.

Author: Xiao-Jia ZHU ¹ ; Yu-Wei WANG ¹ ; Wen-Hui ZHANG ¹ ; Li GAO ¹ ; Yu-Jie XIAO ¹ ; Qian-Wen GAO ¹ ; Rong-Rong WANG ¹ ; Long CHEN ¹
Author Information

1. Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China.
Publication Type:Journal Article
MeSH: Animals; Calcium/metabolism*; Myocardial Contraction; Myocytes, Cardiac/metabolism*; Phosphodiesterase Inhibitors; Phosphoric Diester Hydrolases; Rats; Ryanodine Receptor Calcium Release Channel; Sarcoplasmic Reticulum
From: Acta Physiologica Sinica 2021;73(2):275-285
CountryChina
Language:Chinese
Abstract: This study aimed to explore the positive inotropic effect of phosphodiesterase type 9 (PDE9) inhibitor PF-04449613 in ratsand its cellular and molecular mechanisms. The heart pressure-volume loop (P-V loop) analysis was used to detect the effects of PF-04449613 on rat left ventricular pressure-volume relationship, aortic pressures and peripheral vessel resistance in healthy rats. The Langendorff perfusion of isolated rat heart was used to explore the effects of PF-04449613 on heart contractility. The cardiomyocyte sarcoplasmic reticulum (SR) Ca