Resveratrol inhibits hypoxia-induced oxidative stress and proliferation in pulmonary artery smooth muscle cells through the HIF-1α/NOX4/ROS signaling pathway.
- Author:
Li-Nan HE
1
;
Yu-Ru LAN
1
;
Guang-Ming HE
2
;
Shu-Jin GUO
3
;
Fu-Qiang WEN
1
;
Tao WANG
4
Author Information
1. Laboratory of Pulmonary Diseases, West China Hospital/West China School of Medicine, Sichuan University, Chengdu 610041, China.
2. Department of Critical Care Medicine, Sichuan Cancer Hospital & Institute, Chengdu 610041, China.
3. Sichuan Research Hospital of Translational Medicine, Chinese Academy of Sciences, University of Electronic Science and Technology of China, Chengdu 610054, China.
4. Laboratory of Pulmonary Diseases, West China Hospital/West China School of Medicine, Sichuan University, Chengdu 610041, China. tao.wang@scu.edu.cn.
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Proliferation;
Cells, Cultured;
Hypoxia;
Myocytes, Smooth Muscle;
NADPH Oxidase 4;
Oxidative Stress;
Pulmonary Artery;
Rats;
Reactive Oxygen Species;
Resveratrol/pharmacology*;
Signal Transduction
- From:
Acta Physiologica Sinica
2020;72(5):551-558
- CountryChina
- Language:Chinese
-
Abstract:
The purpose of the present study was to determine the effects of resveratrol on hypoxia-induced oxidative stress and proliferation in pulmonary artery smooth muscle cells (PASMCs) and the underlying mechanism. Primary rat PASMCs were isolated and cultured in vitro and pretreated with different concentrations of resveratrol (10, 20, and 40 µmol/L) or the NADPH oxidase (NOX) inhibitor VAS2870 (10 µmol/L) for 0.5 h. The cells were then cultured under normoxia (21% O
