Effect of Hypoxia on the Survival and Production of Nitric Oxide in Trabecular Meshwork Cells.
- Author:
Yong Hun JIN
1
;
Jae Woo KIM
Author Information
1. Department of Ophthalmology, Dongkang Medical Center, Ulsan, Korea.
- Publication Type:Original Article
- Keywords:
Apoptosis;
Hypoxia;
Nitric oxide;
Trabecular meshwork cells
- MeSH:
Anoxia*;
Apoptosis;
Cell Survival;
Flow Cytometry;
NG-Nitroarginine Methyl Ester;
Nitric Oxide*;
Sodium Cyanide;
Trabecular Meshwork*
- From:Journal of the Korean Ophthalmological Society
2004;45(12):2127-2132
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To investigate the effect of hypoxia on the survival and nitric oxide (NO) production of cultured trabecular meshwork (TM) cells. METHODS: After inducing chemical hypoxia with sodium cyanide, the survival and nitrite production of the primarily cultured porcine TM cells were assessed with MTT and Griess assays. The effect of NOS inhibitor, N(omega)-Nitro-L-arginine methyl ester (L-NAME), was also assessed. Flow cytometry using annexin/PI was done to evaluate apoptosis. RESULTS: Chemical hypoxia decreased TM cell survival significantly (p<0.05) with increased NO production. This hypoxia-induced antiproliferative effect was abolished by L-NAME (p<0.05). Flow cytometric analysis revealed that hypoxia induced apoptosis of TM cells, which was inhibited by L-NAME. CONCLUSIONS: Hypoxia decreases the survival of TM cells and induced apoptosis, accompanied by increased NO production. The hypoxia-induced decreased survival of TM cells may be mediated by NO.
