Genome-wide long non-coding RNA association study on Han Chinese women identifies lncHSAT164 as a novel susceptibility gene for breast cancer.
10.1097/CM9.0000000000001429
- VernacularTitle:Genome-wide long non-coding RNA association study on Han Chinese women identifies lncHSAT164 as a novel susceptibility gene for breast cancer
- Author:
Jing-Kai XU
1
;
Guo-Zheng LI
2
;
Zhi LI
2
;
Wen-Jing LI
2
;
Run-Sheng CHEN
3
;
Bo ZHANG
2
;
Xue-Jun ZHANG
1
Author Information
1. Department of Dermatology, The First Affiliated Hospital of Anhui Medical University, Key Laboratory of Dermatology, Anhui Medical University, Ministry of Education, Hefei, Anhui 230032, China.
2. Department of Genetics, School of Life Sciences, Anhui Medical University, Hefei, Anhui 230032, China.
3. Laboratory of Bioinformatics and Noncoding RNA, Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
- Publication Type:Journal Article
- MeSH:
Asian Continental Ancestry Group/genetics*;
Breast Neoplasms/genetics*;
Case-Control Studies;
China;
Female;
Genetic Predisposition to Disease/genetics*;
Genome-Wide Association Study;
Humans;
Polymorphism, Single Nucleotide/genetics*;
RNA, Long Noncoding/genetics*
- From:
Chinese Medical Journal
2021;134(10):1138-1145
- CountryChina
- Language:English
-
Abstract:
BACKGROUND:Single-nucleotide polymorphisms (SNPs)-associated genes and long non-coding RNAs (lncRNAs) can contribute to human disease. To comprehensively investigate the contribution of lncRNAs to breast cancer, we performed the first genome-wide lncRNA association study on Han Chinese women.
METHODS:We designed an lncRNA array containing >800,000 SNPs, which was incorporated into a 96-array plate by Affymetrix (CapitalBio Technology, China). Subsequently, we performed a two-stage genome-wide lncRNA association study on Han Chinese women covering 11,942 individuals (5634 breast cancer patients and 6308 healthy controls). Additionally, in vitro gain or loss of function strategies were performed to clarify the function of a novel SNP-associated gene.
RESULTS:We identified a novel breast cancer-associated susceptibility SNP, rs11066150 (Pmeta = 2.34 × 10-8), and a previously reported SNP, rs9397435 (Pmeta = 4.32 × 10-38), in Han Chinese women. rs11066150 is located in NONHSAT164009.1 (lncHSAT164), which is highly expressed in breast cancer tissues and cell lines. lncHSAT164 overexpression promoted colony formation, whereas lncHSAT164 knockdown promoted cell apoptosis and reduced colony formation by regulating the cell cycle.
CONCLUSIONS:Based on our lncRNA array, we identified a novel breast cancer-associated lncRNA and found that lncHSAT164 may contribute to breast cancer by regulating the cell cycle. These findings suggest a potential therapeutic target in breast cancer.
